Colloidal polyaniline dispersions: antibacterial activity, cytotoxicity and neutrophil oxidative burst
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24534430
DOI
10.1016/j.colsurfb.2014.01.027
PII: S0927-7765(14)00028-9
Knihovny.cz E-resources
- Keywords
- Antibacterial activity, Apoptosis, Colloidal polyaniline, Cytotoxicity, Necrosis, Oxidative burst,
- MeSH
- Aniline Compounds chemical synthesis chemistry pharmacology MeSH
- Anti-Bacterial Agents chemical synthesis chemistry pharmacology MeSH
- Apoptosis drug effects MeSH
- Bacillus cereus drug effects MeSH
- Cell Line MeSH
- NIH 3T3 Cells MeSH
- Escherichia coli drug effects MeSH
- Colloids chemical synthesis chemistry pharmacology MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Mice MeSH
- Neutrophils drug effects metabolism MeSH
- Surface Properties MeSH
- Pseudomonas aeruginosa drug effects MeSH
- Reactive Oxygen Species metabolism MeSH
- Staphylococcus aureus drug effects MeSH
- Particle Size MeSH
- Cell Survival drug effects MeSH
- Dose-Response Relationship, Drug MeSH
- Structure-Activity Relationship MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Aniline Compounds MeSH
- Anti-Bacterial Agents MeSH
- Colloids MeSH
- polyaniline MeSH Browser
- Reactive Oxygen Species MeSH
Polyaniline colloids rank among promising application forms of this conducting polymer. Cytotoxicity, antibacterial activity, and neutrophil oxidative burst tests were performed on cells treated with colloidal polyaniline dispersions. The antibacterial effect of colloidal polyaniline against gram-positive and gram-negative bacteria was most pronounced for Bacillus cereus and Escherichia coli, with a minimum inhibitory concentration of 3,500 μg mL(-1). The data recorded on human keratinocyte (HaCaT) and a mouse embryonic fibroblast (NIH/3T3) cell lines using an MTT assay and flow cytometry indicated a concentration-dependent cytotoxicity of colloid, with the absence of cytotoxic effect at around 150 μg mL(-1). The neutrophil oxidative burst test then showed that colloidal polyaniline, in concentrations <150 μg mL(-1), was not able to stimulate the production of reactive oxygen species in neutrophils and whole human blood. However, it worked efficiently as a scavenger of those already formed.
Centre of Polymer Systems Tomas Bata University in Zlin T G M Sq 5555 760 01 Zlin Czech Republic
Institute of Biophysics Academy of Sciences of the Czech Republic 612 65 Brno Czech Republic
References provided by Crossref.org
Adhesion, Proliferation and Migration of NIH/3T3 Cells on Modified Polyaniline Surfaces