Both genetic and dietary factors underlie individual differences in DNA damage levels and DNA repair capacity
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24674629
DOI
10.1016/j.dnarep.2014.01.016
PII: S1568-7864(14)00027-5
Knihovny.cz E-resources
- Keywords
- DNA damage, DNA repair capacity, Diet, Genetic polymorphisms, Molecular epidemiology study,
- MeSH
- Antioxidants metabolism MeSH
- DNA-Binding Proteins genetics metabolism MeSH
- Adult MeSH
- Endonucleases genetics metabolism MeSH
- Genetic Variation MeSH
- Genetic Markers MeSH
- Gene-Environment Interaction MeSH
- Nuclear Proteins genetics metabolism MeSH
- Polymorphism, Single Nucleotide MeSH
- Middle Aged MeSH
- Humans MeSH
- Young Adult MeSH
- DNA Repair * MeSH
- DNA Damage genetics MeSH
- Aged MeSH
- Sex Factors MeSH
- Feeding Behavior * MeSH
- Transcription Factors genetics metabolism MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Young Adult MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Norway MeSH
- Names of Substances
- Antioxidants MeSH
- DNA excision repair protein ERCC-5 MeSH Browser
- DNA-Binding Proteins MeSH
- Endonucleases MeSH
- Genetic Markers MeSH
- Nuclear Proteins MeSH
- Transcription Factors MeSH
The interplay between dietary habits and individual genetic make-up is assumed to influence risk of cancer, via modulation of DNA integrity. Our aim was to characterize internal and external factors that underlie inter-individual variability in DNA damage and repair and to identify dietary habits beneficial for maintaining DNA integrity. Habitual diet was estimated in 340 healthy individuals using a food frequency questionnaire and biomarkers of antioxidant status were quantified in fasting blood samples. Markers of DNA integrity were represented by DNA strand breaks, oxidized purines, oxidized pyrimidines and a sum of all three as total DNA damage. DNA repair was characterized by genetic variants and functional activities of base and nucleotide excision repair pathways. Sex, fruit-based food consumption and XPG genotype were factors significantly associated with the level of DNA damage. DNA damage was higher in women (p=0.035). Fruit consumption was negatively associated with the number of all measured DNA lesions, and this effect was mediated mostly by β-cryptoxanthin and β-tocopherol (p<0.05). XPG 1104His homozygotes appeared more vulnerable to DNA damage accumulation (p=0.001). Sex and individual antioxidants were also associated with DNA repair capacity; both the base and nucleotide excision repairs were lower in women and the latter increased with higher plasma levels of ascorbic acid and α-carotene (p<0.05). We have determined genetic and dietary factors that modulate DNA integrity. We propose that the positive health effect of fruit intake is partially mediated via DNA damage suppression and a simultaneous increase in DNA repair capacity.
References provided by Crossref.org
A pooled analysis of host factors that affect nucleotide excision repair in humans
Comet assay to measure DNA repair: approach and applications
