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The Transplantation of hBM-MSCs Increases Bone Neo-Formation and Preserves Hearing Function in the Treatment of Temporal Bone Defects - on the Experience of Two Month Follow Up

Školoudík, Lukáš
Author Školoudík, Lukáš Department of Otorhinolaryngology and Head and Neck Surgery, University Hospital, Hradec Králové, Czech Republic
Chrobok, Viktor
Author Chrobok, Viktor Department of Otorhinolaryngology and Head and Neck Surgery, University Hospital, Hradec Králové, Czech Republic
Kočí, Zuzana
Author Kočí, Zuzana Department of Biomaterials and Biophysical Methods, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic
Popelář, Jiří
Author Popelář, Jiří Department of Auditory Neuroscience, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic
Syka, Josef
Author Syka, Josef Department of Auditory Neuroscience, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic
Laco, Jan
Author Laco, Jan The Fingerland Department of Pathology, University Hospital, Hradec Králové, Czech Republic
Filipová, Alžběta
Author Filipová, Alžběta Department of Radiobiology, University of Defence Brno, Faculty of Military Health Sciences , Hradec Králové, Czech Republic
Syková, Eva
Author Syková, Eva Institute of Neuroimmunology, Slovak Academy of Science, Bratislava, Slovak Republic
Filip, Stanislav
Author Filip, Stanislav Department of Oncology and Radiotherapy, Charles University, Faculty of Medicine, Hradec Králové, Czech Republic. stanfil01@gmail.com

Stem cell reviews and reports. 2018 Dec ; 14 (6) : 860-870.

Stem Cell Rev Rep
ISSN 2629-3277
Source

Language English Country United States Media print

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link https://www.medvik.cz/link/pmid29860618

PubMed 29860618
DOI 10.1007/s12015-018-9831-z
PII: 10.1007/s12015-018-9831-z
Knihovny.cz E-resources

Keywords
Cholesteatoma, Ear function, Mesenchymal stromal cells, Osteogenesis, Scaffold, Temporal bone,
MeSH
Cholesteatoma, Middle Ear * metabolism pathology surgery MeSH
Heterografts MeSH
Humans MeSH
Mastoidectomy adverse effects MeSH
Disease Models, Animal MeSH
Guinea Pigs MeSH
Recovery of Function * MeSH
Osteogenesis * MeSH
Postoperative Complications * metabolism pathology therapy MeSH
Hearing * MeSH
Temporal Bone * injuries metabolism pathology MeSH
Mesenchymal Stem Cell Transplantation * MeSH
Animals MeSH
Check Tag
Humans MeSH
Guinea Pigs MeSH
Male MeSH
Animals MeSH
Publication type
Journal Article MeSH
Research Support, Non-U.S. Gov't MeSH

Temporal bone reconstruction is a persisting problem following middle ear cholesteatoma surgery. Seeking to advance the clinical transfer of stem cell therapy we attempted the reconstruction of temporal bone using a composite bioartificial graft based on a hydroxyapatite bone scaffold combined with human bone marrow-derived mesenchymal stromal cells (hBM-MSCs). The aim of this study was to evaluate the effect of the combined biomaterial on the healing of postoperative temporal bone defects and the preservation of physiological hearing functions in a guinea pig model. The treatment's effect could be observed at 1 and 2 months after implantation of the biomaterial, as opposed to the control group. The clinical evaluation of our results included animal survival, clinical signs of an inflammatory response, and exploration of the tympanic bulla. Osteogenesis, angiogenesis, and inflammation were evaluated by histopathological analyses, whereas hBM-MSCs survival was evaluated by immunofluorescence assays. Hearing capacity was evaluated by objective audiometric methods, i.e. auditory brainstem responses and otoacoustic emission. Our study shows that hBM-MSCs, in combination with hydroxyapatite scaffolds, improves the repair of bone defects providing a safe and effective alternative in their treatment following middle ear surgery due to cholesteatoma.

Department of Auditory Neuroscience Institute of Experimental Medicine Czech Academy of Sciences Prague Czech Republic

Department of Biomaterials and Biophysical Methods Institute of Experimental Medicine Czech Academy of Sciences Prague Czech Republic

Department of Oncology and Radiotherapy Charles University Faculty of Medicine Hradec Králové Czech Republic

Department of Otorhinolaryngology and Head and Neck Surgery University Hospital Hradec Králové Czech Republic

Department of Radiobiology University of Defence Brno Faculty of Military Health Sciences Hradec Králové Czech Republic

Institute of Neuroimmunology Slovak Academy of Science Bratislava Slovak Republic

The Fingerland Department of Pathology University Hospital Hradec Králové Czech Republic

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