Expression levels of elastin and related genes in human varicose veins
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24785109
DOI
10.14712/fb2014060020068
PII: file/5717/FB2014A0009.pdf
Knihovny.cz E-zdroje
- MeSH
- elastin genetika metabolismus MeSH
- extracelulární matrix - proteiny genetika metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- matrixová metaloproteinasa 2 genetika metabolismus MeSH
- oxidoreduktasy aminokyselin genetika metabolismus MeSH
- senioři MeSH
- techniky in vitro MeSH
- varixy MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- elastin MeSH
- extracelulární matrix - proteiny MeSH
- FBLN5 protein, human MeSH Prohlížeč
- LOXL1 protein, human MeSH Prohlížeč
- matrixová metaloproteinasa 2 MeSH
- oxidoreduktasy aminokyselin MeSH
Among the suspected reasons for varicose vein formation are changes in the quantity and content of the elastin protein; however, comprehensive investigations about elastin assembly in varicose vein formation are yet lacking. In this study, we aimed to determine the changes in mRNA levels of elastin and some of its functionally related proteins, fibulin 5, LOXL-1, MMP-2 and MMP-9 in varicose vein formation. We analysed the mRNA levels of elastin, fibulin-5, LOXL1, MMP2 and MMP9 in samples of 35 healthy and 35 varicose great saphenous vein tissues. mRNA levels of these genes were determined by using real-time PCR and normalized with HPRT1. When we compared the patient and control groups, elastin mRNA levels were significantly higher in the patient group than in the control group (P = 0.047), although there were no significant differences in fibulin 5, LOXL1, MMP2 and MMP9 mRNA levels between the patient and control groups. We showed that up-regulation of MMP2 mRNA expression was significantly correlated with hyperlipidaemia (P = 0.029). The up-regulation of elastin expression may play an important role in the pathogenesis of primary varicose veins. Additionally, the up-regulation of MMP2 expression was strongly correlated with hyperlipidaemia in varicose veins.
Istanbul Bilim University Faculty of Medicine Department of Biochemistry Istanbul Turkey
Izmit Seka State Hospital Department of Cardiovascular Surgery Kocaeli Turkey
Marmara University Faculty of Medicine Department of Cardiovascular Surgery Istanbul Turkey
Yeditepe University Faculty of Medicine Department of Medical Biology Istanbul Turkey
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