Expression levels of elastin and related genes in human varicose veins
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24785109
DOI
10.14712/fb2014060020068
PII: file/5717/FB2014A0009.pdf
Knihovny.cz E-resources
- MeSH
- Elastin genetics metabolism MeSH
- Extracellular Matrix Proteins genetics metabolism MeSH
- Middle Aged MeSH
- Humans MeSH
- Matrix Metalloproteinase 2 genetics metabolism MeSH
- Amino Acid Oxidoreductases genetics metabolism MeSH
- Aged MeSH
- In Vitro Techniques MeSH
- Varicose Veins MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Elastin MeSH
- Extracellular Matrix Proteins MeSH
- FBLN5 protein, human MeSH Browser
- LOXL1 protein, human MeSH Browser
- Matrix Metalloproteinase 2 MeSH
- Amino Acid Oxidoreductases MeSH
Among the suspected reasons for varicose vein formation are changes in the quantity and content of the elastin protein; however, comprehensive investigations about elastin assembly in varicose vein formation are yet lacking. In this study, we aimed to determine the changes in mRNA levels of elastin and some of its functionally related proteins, fibulin 5, LOXL-1, MMP-2 and MMP-9 in varicose vein formation. We analysed the mRNA levels of elastin, fibulin-5, LOXL1, MMP2 and MMP9 in samples of 35 healthy and 35 varicose great saphenous vein tissues. mRNA levels of these genes were determined by using real-time PCR and normalized with HPRT1. When we compared the patient and control groups, elastin mRNA levels were significantly higher in the patient group than in the control group (P = 0.047), although there were no significant differences in fibulin 5, LOXL1, MMP2 and MMP9 mRNA levels between the patient and control groups. We showed that up-regulation of MMP2 mRNA expression was significantly correlated with hyperlipidaemia (P = 0.029). The up-regulation of elastin expression may play an important role in the pathogenesis of primary varicose veins. Additionally, the up-regulation of MMP2 expression was strongly correlated with hyperlipidaemia in varicose veins.
Istanbul Bilim University Faculty of Medicine Department of Biochemistry Istanbul Turkey
Izmit Seka State Hospital Department of Cardiovascular Surgery Kocaeli Turkey
Marmara University Faculty of Medicine Department of Cardiovascular Surgery Istanbul Turkey
Yeditepe University Faculty of Medicine Department of Medical Biology Istanbul Turkey
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