Human pluripotent stem cells (hPSCs), namely, embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), with their ability of indefinite self-renewal and capability to differentiate into cell types derivatives of all three germ layers, represent a powerful research tool in developmental biology, for drug screening, disease modelling, and potentially cell replacement therapy. Efficient differentiation protocols that would result in the cell type of our interest are needed for maximal exploitation of these cells. In the present work, we aim at focusing on the protocols for differentiation of hPSCs into functional cardiomyocytes in vitro as well as achievements in the heart disease modelling and drug testing on the patient-specific iPSC-derived cardiomyocytes (iPSC-CMs).

Department of Biology Faculty of Medicine Masaryk University Kamenice 5 A3 62500 Brno Czech Republic

Department of Biology Faculty of Medicine Masaryk University Kamenice 5 A3 62500 Brno Czech Republic ; ICRC St Anne's University Hospital 60200 Brno Czech Republic

Department of Biology Faculty of Medicine Masaryk University Kamenice 5 A3 62500 Brno Czech Republic ; INSERM U1046 University of Montpellier 1 University of Montpellier 2 34295 Montpellier France

INSERM U1046 University of Montpellier 1 University of Montpellier 2 34295 Montpellier France

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Oxygen Is an Ambivalent Factor for the Differentiation of Human Pluripotent Stem Cells in Cardiac 2D Monolayer and 3D Cardiac Spheroids

Dystrophin Deficiency Leads to Genomic Instability in Human Pluripotent Stem Cells via NO Synthase-Induced Oxidative Stress