Lin28a is dormant, functional, and dispensable during mouse oocyte-to-embryo transition
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24829024
DOI
10.1095/biolreprod.114.118703
PII: biolreprod.114.118703
Knihovny.cz E-zdroje
- Klíčová slova
- LIN28, Let-7, RNAi, early development, genome activation, guinea pigs, mice, microRNA, oocyte, rats, rodents, transgenic/knockout model, voles, zygote,
- MeSH
- blastocysta cytologie MeSH
- blastomery cytologie MeSH
- buněčná diferenciace MeSH
- DNA vazebné proteiny genetika fyziologie MeSH
- kultivace embrya MeSH
- luciferasy genetika MeSH
- mikro RNA genetika MeSH
- myši inbrední C57BL MeSH
- myši knockoutované MeSH
- oocyty cytologie MeSH
- přenos embrya metody MeSH
- proteiny vázající RNA genetika fyziologie MeSH
- RNA interference fyziologie MeSH
- RNA messenger skladovaná genetika MeSH
- těhotenství MeSH
- totipotentní kmenové buňky cytologie MeSH
- zvířata MeSH
- zygota cytologie MeSH
- Check Tag
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA vazebné proteiny MeSH
- Lin-28 protein, mouse MeSH Prohlížeč
- Lin28b protein, mouse MeSH Prohlížeč
- luciferasy MeSH
- mikro RNA MeSH
- mirnlet7 microRNA, mouse MeSH Prohlížeč
- proteiny vázající RNA MeSH
- RNA messenger skladovaná MeSH
The oocyte-to-embryo transition (OET) denotes transformation of a highly differentiated oocyte into totipotent blastomeres of the early mammalian embryo. OET depends exclusively on maternal RNAs and proteins accumulated during oocyte growth, which implies importance of post-transcriptional control of gene expression. OET includes replacement of abundant maternal microRNAs (miRNAs), enriched also in differentiated cells and exemplified by the Let-7 family, with embryonic miRNAs common in pluripotent stem cells (the miR-290 family in the mouse). Lin28a and its homolog Lin28b encode RNA-binding proteins, which interfere with Let-7 maturation and facilitate reprogramming of induced pluripotent stem cells. Both Lin28a and Lin28b transcripts are abundant in mouse oocytes. To test the role of maternal expression of Lin28a and Lin28b during oocyte-to-zygote reprogramming, we generated mice with oocyte-specific knockdown of both genes by using transgenic RNA interference. Lin28a and Lin28b are dispensable during oocyte growth because their knockdown has no effect on Let-7a levels in fully grown germinal vesicle (GV)-intact oocytes. Furthermore, transgenic females were fertile and produced healthy offspring, and their overall breeding performance was comparable to that of wild-type mice. At the same time, 2-cell embryos derived from transgenic females showed up-regulation of mature Let-7, suggesting that maternally provided LIN28A and LIN28B function during zygotic genome activation. Consistent with this conclusion is increased translation of Lin28a transcripts upon resumption of meiosis. Our data imply dual repression of Let-7 during OET in the mouse model, the selective suppression of Let-7 biogenesis by Lin28 homologs superimposed on previously reported global suppression of miRNA activity.
Citace poskytuje Crossref.org
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