Evidence of passive faecal shedding of Mycobacterium avium subsp. paratuberculosis in a Limousin cattle herd
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24836889
DOI
10.1016/j.tvjl.2014.02.011
PII: S1090-0233(14)00065-3
Knihovny.cz E-zdroje
- Klíčová slova
- Cattle, Culture, Paratuberculosis, Passive shedding, qPCR,
- MeSH
- ELISA veterinární MeSH
- feces mikrobiologie MeSH
- kvantitativní polymerázová řetězová reakce veterinární MeSH
- Mycobacterium avium subsp. paratuberculosis genetika izolace a purifikace MeSH
- nemoci skotu epidemiologie mikrobiologie MeSH
- paratuberkulóza epidemiologie mikrobiologie MeSH
- prevalence MeSH
- roční období MeSH
- skot MeSH
- vylučování bakterií z těla * MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Francie epidemiologie MeSH
It has been suggested that passive shedding of Mycobacterium avium subsp. paratuberculosis (MAP) in faeces may occur, but reliable data are missing. Passive shedding assumes the ingestion of MAP in contaminated feed and passive passage through the gastrointestinal tract without causing infection. In this study the presence of MAP in faeces in a closed herd of Limousin cattle was monitored for 53 months using quantitative real time PCR (qPCR) and culture. The initial prevalence of MAP in the herd was determined to be 63.4% and 4.9% using qPCR and culture, respectively. After the removal of two culture- and qPCR-positive (>10(4) MAP cells/g) cows, the prevalence of MAP using qPCR decreased to 42.1% and later to 15.6% and 6.7%. The continuous removal of suspected animals from the herd during the monitoring period minimised the presence of MAP in faeces to sporadic, which may have resulted from a decrease in the environmental infectious pressure. The findings suggest that the presence of low numbers of MAP in bovine faeces may not necessarily be caused by real infection, but rather by passive passage of MAP. This phenomenon should therefore be considered when interpreting MAP qPCR data.
Citace poskytuje Crossref.org
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