Relationship of mismatch repair proteins and survivin in colon polyps and carcinomas
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24852932
DOI
10.1016/j.acthis.2014.04.005
PII: S0065-1281(14)00072-5
Knihovny.cz E-resources
- Keywords
- Colon carcinomas, Colon polyps, Mismatch repair proteins, Survivin,
- MeSH
- Adaptor Proteins, Signal Transducing metabolism MeSH
- Adenocarcinoma enzymology MeSH
- Adenosine Triphosphatases metabolism MeSH
- DNA-Binding Proteins metabolism MeSH
- DNA Repair Enzymes metabolism MeSH
- MutS Homolog 2 Protein metabolism MeSH
- Inhibitor of Apoptosis Proteins metabolism MeSH
- Nuclear Proteins metabolism MeSH
- Middle Aged MeSH
- Humans MeSH
- Mismatch Repair Endonuclease PMS2 MeSH
- MutL Protein Homolog 1 MeSH
- MutL Proteins MeSH
- Neoplasm Proteins metabolism MeSH
- Colonic Neoplasms enzymology MeSH
- DNA Mismatch Repair MeSH
- Colonic Polyps enzymology MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Survivin MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adaptor Proteins, Signal Transducing MeSH
- Adenosine Triphosphatases MeSH
- BIRC5 protein, human MeSH Browser
- DNA-Binding Proteins MeSH
- DNA Repair Enzymes MeSH
- G-T mismatch-binding protein MeSH Browser
- MutS Homolog 2 Protein MeSH
- Inhibitor of Apoptosis Proteins MeSH
- Nuclear Proteins MeSH
- Mismatch Repair Endonuclease PMS2 MeSH
- MLH1 protein, human MeSH Browser
- MSH2 protein, human MeSH Browser
- MutL Protein Homolog 1 MeSH
- MutL Proteins MeSH
- Neoplasm Proteins MeSH
- PMS1 protein, human MeSH Browser
- PMS2 protein, human MeSH Browser
- Survivin MeSH
Mismatch repair genes (MMR) play an essential role in DNA repair. MMR mutations predominantly in MLH1, MSH2, MSH6, PMS2, and rarely in PMS1, may cause the production of abnormally short or inactivated proteins. The antiapoptotic protein survivin functions in the inhibition of apoptosis, regulation of cell division and also enhances angiogenesis. Both MMRP and survivin are considered to be powerful prognostic parameters. This study was designed to determine the relationship between MMRP and survivin in colon lesions. The study included 113 cases of colon carcinoma and 51 cases of colon polyps. Survivin expression and MMRP status were assessed by immunohistochemistry. In each section, expression, intensity of immunostaining and percentage of labeled cells were analyzed. In carcinomas, immunoreaction was detected in 100/113 cases for MLH1 (88.5%), 112/113 cases for MSH2 (99.1%), 110/113 cases for MSH6 (97.3%), and 103/113 cases for PMS2 (91.2%). Survivin was shown in 47/113 cases (41.6%). The statistical analysis confirmed a significant correlation between the expression of MMRP and survivin in the assessed parameters. All 51 polyp samples were positive for MLH1, MSH2, MSH6 and PMS2. Only 8 of those (15.7%) were positive for survivin. Statistically significant differences were observed between the expression of MMRP and survivin. In conclusion, this study revealed that MMRP may suppress the antiapoptotic function of survivin through p53 inactivation of its promoter in grade 1 and grade 2 colon carcinomas.
Department of Pathology Faculty of Medicine OU Syllabova 19 703 00 Ostrava Czech Republic
Laboratory of Pathological Anatomy Alpha Medical a s Hodžova 1 03601 Martin Slovakia
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