6S RNA modulates growth and antibiotic production in Streptomyces coelicolor
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Anti-Bacterial Agents biosynthesis MeSH
- RNA, Bacterial genetics metabolism MeSH
- Gene Deletion MeSH
- DNA-Directed RNA Polymerases metabolism MeSH
- RNA, Untranslated genetics metabolism MeSH
- Gene Expression Regulation, Bacterial * MeSH
- Streptomyces coelicolor genetics growth & development metabolism MeSH
- Genetic Complementation Test MeSH
- Protein Binding MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 6S RNA MeSH Browser
- Anti-Bacterial Agents MeSH
- RNA, Bacterial MeSH
- DNA-Directed RNA Polymerases MeSH
- RNA, Untranslated MeSH
- RNA polymerase beta subunit MeSH Browser
The aim of this study was to contribute to clarifying the role of 6S RNA in the development and control of antibiotic biosynthesis in Streptomyces coelicolor. Due to the low energetic cost of gene silencing via 6S RNA, it is an easy and rapid means of down-regulating the expression of specific genes in response to signals from changes in the environment. The expression of 6S RNA in S. coelicolor is not constitutive, and its accumulation is adapted to changes in nutritional conditions. The 6S RNA of S. coelicolor is capable of interacting with RNA polymerase β β' subunits and is a template for the transcription of short pRNAs. Deletion of the ssrS gene from S. coelicolor affects the growth rate and causes changes in the expression of several pathway-specific genes involved in actinorhodin biosynthesis. The complementation of the ΔssrS strain with ssrS gene restored the wild-type levels of growth and actinorhodin production. We conclude that 6S RNA contributes to the optimization of cellular adaptation and is an important factor involved in the regulation of growth and expression of key genes for the biosynthesis of actinorhodin.
References provided by Crossref.org
RIP-seq reveals RNAs that interact with RNA polymerase and primary sigma factors in bacteria
Polyenic Antibiotics and Other Antifungal Compounds Produced by Hemolytic Streptomyces Species
6S-Like scr3559 RNA Affects Development and Antibiotic Production in Streptomyces coelicolor
RNase III-Binding-mRNAs Revealed Novel Complementary Transcripts in Streptomyces
