Differentially expressed proteins in human breast cancer cells sensitive and resistant to paclitaxel
Jazyk angličtina Země Řecko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24898082
DOI
10.3892/ijo.2014.2484
Knihovny.cz E-zdroje
- MeSH
- 2D gelová elektroforéza MeSH
- adenokarcinom metabolismus MeSH
- antigeny nádorové biosyntéza MeSH
- antitumorózní látky fytogenní farmakologie MeSH
- chemorezistence fyziologie MeSH
- keratin-18 biosyntéza MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- nádory prsu metabolismus MeSH
- paclitaxel farmakologie MeSH
- proteiny tepelného šoku HSP27 biosyntéza MeSH
- proteomika MeSH
- serpiny biosyntéza MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- western blotting MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigeny nádorové MeSH
- antitumorózní látky fytogenní MeSH
- keratin-18 MeSH
- paclitaxel MeSH
- proteiny tepelného šoku HSP27 MeSH
- serpiny MeSH
- squamous cell carcinoma-related antigen MeSH Prohlížeč
The resistance of cancer cells to chemotherapeutic drugs represents a major problem in cancer treatment. Despite all efforts, mechanisms of resistance have not yet been elucidated. To reveal proteins that could be involved in resistance to taxanes, we compared protein expression in whole cell lysates of SK-BR-3 breast cancer cells sensitive to paclitaxel and in lysates of the same line with acquired resistance to paclitaxel. The resistant SK-BR-3 cell line was established in our lab. Protein separation was achieved using high-resolution 2D-electrophoresis, computer analysis and mass spectro-metry. With these techniques we identified four proteins with different expression in resistant SK-BR-3 cells, i.e., serpin B3, serpin B4, heat shock protein 27 (all three upregulated) and cytokeratin 18 (downregulated). Observed changes were confirmed using western blot analysis. This study suggests new directions worthy of further study in the effort to reveal the mechanism of resistance to paclitaxel in breast cancer cells.
Citace poskytuje Crossref.org
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