Differentially expressed proteins in human breast cancer cells sensitive and resistant to paclitaxel
Language English Country Greece Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24898082
DOI
10.3892/ijo.2014.2484
Knihovny.cz E-resources
- MeSH
- Electrophoresis, Gel, Two-Dimensional MeSH
- Adenocarcinoma metabolism MeSH
- Antigens, Neoplasm biosynthesis MeSH
- Antineoplastic Agents, Phytogenic pharmacology MeSH
- Drug Resistance, Neoplasm physiology MeSH
- Keratin-18 biosynthesis MeSH
- Humans MeSH
- Cell Line, Tumor MeSH
- Breast Neoplasms metabolism MeSH
- Paclitaxel pharmacology MeSH
- HSP27 Heat-Shock Proteins biosynthesis MeSH
- Proteomics MeSH
- Serpins biosynthesis MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
- Blotting, Western MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Neoplasm MeSH
- Antineoplastic Agents, Phytogenic MeSH
- Keratin-18 MeSH
- Paclitaxel MeSH
- HSP27 Heat-Shock Proteins MeSH
- Serpins MeSH
- squamous cell carcinoma-related antigen MeSH Browser
The resistance of cancer cells to chemotherapeutic drugs represents a major problem in cancer treatment. Despite all efforts, mechanisms of resistance have not yet been elucidated. To reveal proteins that could be involved in resistance to taxanes, we compared protein expression in whole cell lysates of SK-BR-3 breast cancer cells sensitive to paclitaxel and in lysates of the same line with acquired resistance to paclitaxel. The resistant SK-BR-3 cell line was established in our lab. Protein separation was achieved using high-resolution 2D-electrophoresis, computer analysis and mass spectro-metry. With these techniques we identified four proteins with different expression in resistant SK-BR-3 cells, i.e., serpin B3, serpin B4, heat shock protein 27 (all three upregulated) and cytokeratin 18 (downregulated). Observed changes were confirmed using western blot analysis. This study suggests new directions worthy of further study in the effort to reveal the mechanism of resistance to paclitaxel in breast cancer cells.
References provided by Crossref.org
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