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C. difficile ribotype 027 or 176?

Krutova, Marcela
Author Krutova, Marcela Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital, V Uvalu 84, 150 06, Prague 5, Czech Republic, marcela.krutova@seznam.cz
Matejkova, Jana
Author Matejkova, Jana
Nyc, Otakar
Author Nyc, Otakar

Folia microbiologica. 2014 Nov ; 59 (6) : 523-6. [epub] 20140627

Folia Microbiol (Praha)
ISSN 1874-9356 | 0015-5632
Source

Language English Country United States Media print-electronic

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link https://www.medvik.cz/link/pmid24970104

PubMed 24970104
DOI 10.1007/s12223-014-0323-5
Knihovny.cz E-resources

MeSH
Bacterial Proteins genetics MeSH
Clostridioides difficile classification genetics isolation & purification MeSH
Gene Deletion MeSH
Feces microbiology MeSH
Cross Infection microbiology MeSH
Humans MeSH
Hospitals statistics & numerical data MeSH
Ribotyping MeSH
Check Tag
Humans MeSH
Publication type
Journal Article MeSH
Research Support, Non-U.S. Gov't MeSH
Geographicals
Czech Republic MeSH
Names of Substances
Bacterial Proteins MeSH

Clostridium difficile is a major nosocomial pathogen of present times. The analysis of 624 C. difficile strains from 11 hospitals in the Czech Republic in 2013 revealed that 40% of isolates belonged to ribotype 176. These results suggest that the incidence of CDI (C. difficile infection) in the Czech Republic has increased probably in connection with C. difficile ribotype 176. The molecular systems Xpert C. difficile Epi assay (Cepheid Inc., Sunnyvale, CA) diagnoses toxigenic strains and supports C. difficile ribotype 027 determination based on three specific target places in the toxigenic C. difficile genome. Twenty-nine strains cultivated from stool specimens were evaluated by the Xpert systems as presumed C. difficile PCR ribotype 027 were confirmed as a C. difficile ribotype 176 based on ribotyping. A further 120 C. difficile strains of ribotype 176 were examined for the presence of genes tcdB, cdtB and deletion in position 117 in the tcdC gene. Our experience shows that due to the correspondence of the target places, C. difficile ribotype 176 may be interpreted as ribotype 027 by Xpert C. difficile Epi assay (Cepheid Inc., Sunnyvale, CA). Further molecular analysis as ribotyping based on capillary electrophoresis is needed to differentiate between C. difficile ribotypes 027 and 176 for appropriate epidemiological situation control on local and national levels.

Department of Medical Microbiology 2nd Faculty of Medicine Charles University Prague and Motol University Hospital 5 Uvalu 84 150 06 Prague 5 Czech Republic

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