Molecular typing and antimicrobial susceptibility testing to six antimicrobials of Clostridium difficile isolates from three Czech hospitals in Eastern Bohemia in 2011-2012

. 2017 Sep ; 62 (5) : 445-451. [epub] 20170322

Jazyk angličtina Země Spojené státy americké Médium print-electronic

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/pmid28332133
Odkazy

PubMed 28332133
DOI 10.1007/s12223-017-0515-x
PII: 10.1007/s12223-017-0515-x
Knihovny.cz E-zdroje

In 2011-2012, a survey was performed in three regional hospitals in the Czech Republic to determine the incidence of Clostridium difficile infections (CDIs) and to characterize bacterial isolates. C. difficile isolates were characterized by PCR ribotyping, toxin genes detection, multiple-locus variable-number tandem-repeat analysis (MLVA), and antimicrobial susceptibility testing to fidaxomicin, vancomycin, metronidazole, clindamycin, LFF571, and moxifloxacin using agar dilution method. The incidence of CDI in three studied hospitals was 145, 146, and 24 cases per 100,000 inhabitants in 2011 and 177, 258, and 67 cases per 100,000 inhabitants in 2012. A total of 64 isolates of C. difficile was available for molecular typing and antimicrobial susceptibility testing. 60.9% of the isolates were classified as ribotype 176. All 41 isolates of ribotypes 176 and 078 were positive for the presence of binary toxin genes. Ribotype 176 also carried 18-bp deletion in the regulatory gene tcdC. Tested isolates of C. difficile were fully susceptible to vancomycin and metronidazole, whereas 65.1% of the isolates were resistant to moxifloxacin. MLVA results indicated that isolates from three different hospitals were genetically related, suggesting transmission between healthcare facilities.

Zobrazit více v PubMed

Gut Liver. 2014 Jan;8(1):1-6 PubMed

Ann Clin Microbiol Antimicrob. 2015 Dec 23;14:55 PubMed

Mol Microbiol. 2007 Jun;64(5):1274-88 PubMed

Eur J Clin Microbiol Infect Dis. 2008 Dec;27(12):1201-7 PubMed

Anaerobe. 2015 Feb;31:37-41 PubMed

Antimicrob Agents Chemother. 2000 Feb;44(2):411-3 PubMed

Folia Microbiol (Praha). 2014 Nov;59(6):523-6 PubMed

Nat Rev Microbiol. 2009 Jul;7(7):526-36 PubMed

J Clin Microbiol. 2002 Sep;40(9):3470-5 PubMed

J Clin Microbiol. 2007 Mar;45(3):1024-8 PubMed

Lancet. 2011 Apr 23;377(9775):1407 PubMed

Nat Microbiol. 2016 Jul 11;1(8):16108 PubMed

Anaerobe. 2009 Dec;15(6):237-40 PubMed

Clin Microbiol Infect. 2015 Mar;21(3):248.e9-248.e16 PubMed

J Med Microbiol. 2012 Jan;61(Pt 1):49-56 PubMed

J Clin Microbiol. 1999 Feb;37(2):461-3 PubMed

Clin Infect Dis. 2012 Oct;55(8):1056-63 PubMed

J Med Microbiol. 2008 Jun;57(Pt 6):702-8 PubMed

Clin Microbiol Infect. 2007 Nov;13(11):1058-64 PubMed

FEMS Microbiol Lett. 1999 Jun 15;175(2):261-6 PubMed

Folia Microbiol (Praha). 2014 May;59(3):209-15 PubMed

Infection. 2007 Oct;35(5):300-7 PubMed

J Clin Microbiol. 2006 Jul;44(7):2558-66 PubMed

Anaerobe. 2015 Dec;36:88-90 PubMed

J Gastrointest Surg. 2013 Jan;17(1):118-24; discussion p.124-5 PubMed

Lancet. 2011 Jan 1;377(9759):63-73 PubMed

Antimicrob Agents Chemother. 2014 Jul;58(7):4214-8 PubMed

Anaerobe. 2014 Aug;28:13-7 PubMed

Dtsch Arztebl Int. 2014 Oct 24;111(43):723-31 PubMed

Clin Microbiol Infect. 2008 Nov;14(11):1057-64 PubMed

Clin Infect Dis. 2008 Nov 1;47(9):1162-70 PubMed

PLoS One. 2012;7(8):e43247 PubMed

J Antimicrob Chemother. 2015;70(6):1751-6 PubMed

J Antimicrob Chemother. 2013 Jun;68(6):1305-11 PubMed

Anaerobe. 2016 Dec;42:142-144 PubMed

PLoS One. 2015 Feb 13;10(2):e0118150 PubMed

Anaerobe. 2016 Aug;40:35-40 PubMed

Najít záznam

Citační ukazatele

Nahrávání dat ...

Možnosti archivace

Nahrávání dat ...