Clostridium difficile ribotype 176 - A predictor for high mortality and risk of nosocomial spread?
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
27155489
DOI
10.1016/j.anaerobe.2016.05.002
PII: S1075-9964(16)30054-3
Knihovny.cz E-zdroje
- Klíčová slova
- ATLAS score, Clostridium difficile, MLVA, PCR-Ribotype 176, Ribotyping, Thr82Ile,
- MeSH
- analýza přežití MeSH
- antibakteriální látky terapeutické užití MeSH
- bakteriální léková rezistence genetika MeSH
- bakteriální proteiny genetika MeSH
- Clostridioides difficile klasifikace účinky léků genetika izolace a purifikace MeSH
- dítě MeSH
- dospělí MeSH
- exprese genu MeSH
- infekce spojené se zdravotní péčí diagnóza farmakoterapie mortalita patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- multilokusová sekvenční typizace MeSH
- mutace MeSH
- předškolní dítě MeSH
- průjem diagnóza farmakoterapie mortalita patologie MeSH
- pseudomembranózní enterokolitida diagnóza farmakoterapie mortalita patologie MeSH
- retrospektivní studie MeSH
- ribotypizace MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- stupeň závažnosti nemoci MeSH
- výsledek terapie MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky MeSH
- bakteriální proteiny MeSH
PURPOSE: The objective of this survey was to determine the incidence of Clostridium difficile infections (CDI) at the Department of Infectious Diseases, Bulovka Hospital, and to evaluate clinical and epidemiological data on CDI patients together with a detailed molecular characterisation of C. difficile isolates. The patient outcomes were correlated to causative C. difficile PCR-ribotype. METHODS: The twelve-month study (2013) comprised patients two years of age and older with CDI. CDI severity was estimated using ESCMID criteria and ATLAS scoring. C. difficile isolates were further characterized using ribotyping, Multiple-Locus Variable Tandem-Repeats analysis (MLVA) and investigation of antibiotic-resistance determinants (gyrA, gyrB, rpoB, ermB). RESULTS: A total of 619 diarrhoeal stools were investigated. Seventy-two stool samples were GDH and toxin A/B positive, and 39 samples were GDH positive only and subsequently toxigenic C. difficile was cultured. In total, 111 C. difficile isolates were characterized, of which 64 (57.7%) belonged to PCR-ribotype 176. MLVA analysis of PCR-ribotype 176 isolates revealed 11 clonal complexes. Seventy-two isolates (64.9%) showed amino acid substitution Thr82Ile in the GyrA, and sixty-two isolates (55.9%) showed amino acid substitutions Arg505Lys together with His502Asn, or Asp492Glu together with Arg505Lys in the RpoB. Twelve isolates (10.8%) were ermB positive. Severe CDI according to the ESCMID criteria was recorded in forty-two patients (37.8%), and sixteen patients (14.4%) had ATLAS score ≥ 6. Twenty-nine patients (26.1%) had recurrent CDI and twenty-four patients (21.6%) died during the study period. CONCLUSIONS: A higher rate of severe CDI, recurrences and mortality in association with PCR-ribotype 176 infections were observed. The high incidence of PCR-ribotype 176 in the study, and the presence of clonal relatedness between PCR-ribotype 176 isolates, indicate its higher capacity to spread in a hospital setting, which in turn highlights the need to implement strict epidemic measures when PCR-ribotype 176 occurs.
Citace poskytuje Crossref.org
