Enhanced multiparametric hyaluronan degradation for production of molar-mass-defined fragments
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25129744
DOI
10.1016/j.carbpol.2014.05.096
PII: S0144-8617(14)00576-1
Knihovny.cz E-resources
- Keywords
- Hyaluronan fragmentation, Magnetic particles, Papain, Pharmaceutics, SEC-MALS,
- MeSH
- Cellulose chemistry MeSH
- Enzymes, Immobilized chemistry MeSH
- Chromatography, Gel MeSH
- Hyaluronoglucosaminidase chemistry MeSH
- Hyaluronic Acid chemistry MeSH
- Magnetic Phenomena MeSH
- Molecular Weight MeSH
- Native Polyacrylamide Gel Electrophoresis MeSH
- Papain chemistry MeSH
- Scattering, Radiation MeSH
- Viscosity MeSH
- Iron chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cellulose MeSH
- Enzymes, Immobilized MeSH
- Hyaluronoglucosaminidase MeSH
- Hyaluronic Acid MeSH
- Papain MeSH
- Iron MeSH
Hyaluronic acid (HA) is known to serve as a dynamic mediator intervening in many physiological functions. Its specific effect has been repeatedly confirmed to be strongly influenced by the molecular size of hyaluronan fragments. However common technological approaches of HA fragments production have their limitations. In many cases, the final products do not meet the strict pharmaceutical requirements, specifically due to size polydispersity and reaction contaminants. We present novel methodology based on combination of unique incidental ability of the plant-derived protease papain to split the glycosidic bonds and an indispensable advantages of biocompatible macroporous material with incorporated ferrous ions serving as carrier for covalent papain fixation. This atypical and yet unpublished highly efficient multiparametric approach allows enhanced HA fragmentation for easily and safely producing molar-mass-defined HA fragments with narrow size distribution. Native polyacrylamide gel electrophoresis (PAGE) and size exclusion chromatography/multi-angle light scattering (SEC-MALS) confirmed the effectiveness of our multiparametric approach.
References provided by Crossref.org
Hyaluronic Acid: Known for Almost a Century, but Still in Vogue
