Enhanced multiparametric hyaluronan degradation for production of molar-mass-defined fragments
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25129744
DOI
10.1016/j.carbpol.2014.05.096
PII: S0144-8617(14)00576-1
Knihovny.cz E-zdroje
- Klíčová slova
- Hyaluronan fragmentation, Magnetic particles, Papain, Pharmaceutics, SEC-MALS,
- MeSH
- celulosa chemie MeSH
- enzymy imobilizované chemie MeSH
- gelová chromatografie MeSH
- hyaluronoglukosaminidasa chemie MeSH
- kyselina hyaluronová chemie MeSH
- magnetické jevy MeSH
- molekulová hmotnost MeSH
- nativní elektroforéza na polyakrylamidovém gelu MeSH
- papain chemie MeSH
- radiační rozptyl MeSH
- viskozita MeSH
- železo chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- celulosa MeSH
- enzymy imobilizované MeSH
- hyaluronoglukosaminidasa MeSH
- kyselina hyaluronová MeSH
- papain MeSH
- železo MeSH
Hyaluronic acid (HA) is known to serve as a dynamic mediator intervening in many physiological functions. Its specific effect has been repeatedly confirmed to be strongly influenced by the molecular size of hyaluronan fragments. However common technological approaches of HA fragments production have their limitations. In many cases, the final products do not meet the strict pharmaceutical requirements, specifically due to size polydispersity and reaction contaminants. We present novel methodology based on combination of unique incidental ability of the plant-derived protease papain to split the glycosidic bonds and an indispensable advantages of biocompatible macroporous material with incorporated ferrous ions serving as carrier for covalent papain fixation. This atypical and yet unpublished highly efficient multiparametric approach allows enhanced HA fragmentation for easily and safely producing molar-mass-defined HA fragments with narrow size distribution. Native polyacrylamide gel electrophoresis (PAGE) and size exclusion chromatography/multi-angle light scattering (SEC-MALS) confirmed the effectiveness of our multiparametric approach.
Citace poskytuje Crossref.org
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