Monodisperse carboxyl-functionalized poly(ethylene glycol)-coated magnetic poly(glycidyl methacrylate) microspheres: application to the immunocapture of β-amyloid peptides
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- CE-LIF detection, functionalization, magnetic, microspheres, β-amyloid peptides,
- MeSH
- Adsorption MeSH
- Amyloid beta-Peptides isolation & purification MeSH
- Chromatography, Affinity MeSH
- Electrophoresis, Capillary MeSH
- Immunoglobulin G metabolism MeSH
- Carboxylic Acids chemistry MeSH
- Polymethacrylic Acids chemistry MeSH
- Magnetic Phenomena MeSH
- Microspheres * MeSH
- Microscopy, Atomic Force MeSH
- Peptide Fragments isolation & purification MeSH
- Polyethylene Glycols chemistry MeSH
- Cattle MeSH
- Thermogravimetry MeSH
- Particle Size MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- amyloid beta-protein (1-40) MeSH Browser
- Amyloid beta-Peptides MeSH
- Immunoglobulin G MeSH
- Carboxylic Acids MeSH
- Polymethacrylic Acids MeSH
- Peptide Fragments MeSH
- Polyethylene Glycols MeSH
- polyglycidyl methacrylate MeSH Browser
Identification and evaluation of small changes in β-amyloid peptide (Aβ) levels in cerebrospinal fluid is of crucial importance for early detection of Alzheimer's disease. Microfluidic detection methods enable effective preconcentration of Aβ using magnetic microparticles coated with Aβ antibodies. Poly(glycidyl methacrylate) microspheres are coated with α-amino-ω-methoxy-PEG5000 /α-amino-ω-Boc-NH-PEG5000 Boc groups deprotected and NH2 succinylated to introduce carboxyl groups. Capillary electrophoresis with laser-induced fluorescence detection confirms the efficient capture of Aβ 1-40 peptides on the microspheres with immobilized monoclonal anti-Aβ 6E10. The capture specificity is confirmed by comparing Aβ 1-40 levels on the anti-IgG-immobilized particles used as a control.
References provided by Crossref.org
Magnetic Nanoparticles: From Design and Synthesis to Real World Applications
