B cell subsets are activated and produce cytokines during early phases of Francisella tularensis LVS infection
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25200734
DOI
10.1016/j.micpath.2014.08.009
PII: S0882-4010(14)00123-5
Knihovny.cz E-resources
- Keywords
- Activating markers, Antigen-presenting function, B cells, Cytokines, F. tularensis,
- MeSH
- Lymphocyte Activation * MeSH
- Time Factors MeSH
- Antigens, CD analysis MeSH
- Cytokines metabolism MeSH
- Histocompatibility Antigens Class II analysis MeSH
- Disease Models, Animal MeSH
- Mice, Inbred BALB C MeSH
- Peritoneum immunology MeSH
- B-Lymphocyte Subsets chemistry immunology MeSH
- Spleen immunology MeSH
- Tularemia immunology MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, CD MeSH
- Cytokines MeSH
- Histocompatibility Antigens Class II MeSH
Francisella tularensis, a facultative intracellular Gram-negative bacterium, causes the illness tularemia. The infection of mice with live vaccine strain is considered to be a model of human tularemia. F. tularensis infects predominantly such phagocytic cells as macrophages or neutrophils, but it also infects non-phagocytic hepatocytes, epithelial cells, and murine and human B cell lines. Based on work with the murine tularemia model, we report here that F. tularensis LVS infects peritoneal CD19(+) cells - exclusively B-1a cells - early after intraperitoneal infection in vivo. The peritoneal and consequently spleen CD19(+) cells are activated by the F. tularensis LVS infection to express the activation markers from MHC class II, CD25, CD54, CD69, and the co-stimulatory molecules CD80 and CD86. As early as 12 h post-infection, the peritoneal CD19(+) cells produce IFN-γ, IL-1β, IL-4, IL-6, IL-12, IL-17, IL-23, and TNF-α. The spleen CD19(+) cells respond to infection with some delay. Moreover, the F. tularensis infected A20 B cell line activates CD3(+) spleen cells isolated from naïve mice. Thus, the data presented here suggest that B cells have all the attributes to actively participate in the induction and regulation of the adaptive immune response during early stages of F. tularensis infection.
Centre of Advanced Studies FMHS UO Hradec Kralove Czech Republic
Department of Radiobiology FMHS UO Hradec Kralove Czech Republic
References provided by Crossref.org
Early infection-induced natural antibody response