Zoonotic Dirofilaria repens (Nematoda: Filarioidea) in Aedes vexans mosquitoes, Czech Republic
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Aedes classification parasitology MeSH
- Dirofilaria repens genetics isolation & purification MeSH
- Dirofilariasis epidemiology parasitology transmission MeSH
- Insect Vectors classification parasitology MeSH
- Humans MeSH
- Molecular Sequence Data MeSH
- Dog Diseases epidemiology parasitology transmission MeSH
- Polymerase Chain Reaction veterinary MeSH
- Dogs MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA veterinary MeSH
- Fresh Water MeSH
- Public Health MeSH
- Geography MeSH
- Zoonoses parasitology transmission MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Dogs MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic epidemiology MeSH
The surveillance of vectors for arthropod-borne pathogens is nowadays an important tool in surveillance programmes throughout Europe. Whereas many studies have been performed to screen arthropods for viruses or bacterial pathogens, only limited information is available concerning the geographical distribution and vector range of pathogenic filariae in Central Europe. To consider the prevalence of filarial parasites in mosquito vectors, we performed a molecular survey of mosquitoes for filarial DNA. Mosquito collection was conducted at six study sites in the South Moravian region (Czech Republic) close to the borders with Slovakia and Austria from 2009 to 2011. Molecular screening of mosquitoes was conducted using conventional PCR with primers designed to amplify the mitochondrial cytochromoxidase subunit I gene as well as the partial 5.8S ribosomal RNA gene. A total of 13,222 mosquitoes belonging to six species were captured and distributed into 237 pools with different numbers of individuals. Overall, four pools were positive for Dirofilaria repens (a minimum infection rate 0.03%) at two study sites (both natural and urban). Another filarial parasite detected during a study into Aedes vexans mosquitoes revealed the closest homology to Setaria spp. We detected specific D. repens DNA in Ae. vexans mosquitoes for the first time in the Czech Republic and confirmed the circulation of Dirofilaria spp. in a natural focus of infection providing an epidemiological link between autochthonous canine cases and mosquito vectors in the area studied.
See more in PubMed
J Helminthol. 2013 Mar;87(1):85-90 PubMed
Parassitologia. 2000 Dec;42(3-4):231-54 PubMed
Parasit Vectors. 2014 Jan 16;7:30 PubMed
Parasitol Res. 2013 Oct;112(10):3465-70 PubMed
Vet Parasitol. 2006 Feb 18;135(3-4):303-14 PubMed
Ann Agric Environ Med. 2012;19(3):445-50 PubMed
Parasit Vectors. 2014 May 14;7:226 PubMed
Parassitologia. 1995 Dec;37(2-3):149-93 PubMed
Euro Surveill. 2014 May 01;19(17):2-4 PubMed
Emerg Infect Dis. 2014 Feb;20(2):328-31 PubMed
The first case of periorbital human dirofilariasis in the Czech Republic
Cryptic species Anopheles daciae (Diptera: Culicidae) found in the Czech Republic and Slovakia
Mosquitoes in the Danube Delta: searching for vectors of filarioid helminths and avian malaria
Dirofilaria repens: emergence of autochthonous human infections in the Czech Republic (case reports)
GENBANK
KM108773, KM108774, KM108775
