17β-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line
Jazyk angličtina Země Řecko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25434399
DOI
10.3892/or.2014.3627
Knihovny.cz E-zdroje
- MeSH
- antitumorózní látky chemie MeSH
- estradiol metabolismus MeSH
- genetická terapie metody MeSH
- glutathion chemie MeSH
- koncentrace vodíkových iontů MeSH
- kyslík chemie MeSH
- lidé MeSH
- liposomy chemie MeSH
- malondialdehyd chemie MeSH
- metalothionein chemie MeSH
- MFC-7 buňky MeSH
- mikroskopie fázově kontrastní MeSH
- nádorové buněčné linie MeSH
- nádory prsu metabolismus MeSH
- oxidace-redukce MeSH
- oxidační stres MeSH
- proliferace buněk MeSH
- receptory pro estrogeny metabolismus MeSH
- regulace genové exprese MeSH
- systémy cílené aplikace léků * MeSH
- viabilita buněk MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antitumorózní látky MeSH
- estradiol MeSH
- glutathion MeSH
- kyslík MeSH
- liposomy MeSH
- malondialdehyd MeSH
- metalothionein MeSH
- receptory pro estrogeny MeSH
The present study suggests and describes the application of a delivery system for antisense oligonucleotides against mRNA encoding estrogen receptor proteins α and β. The delivery system is composed of a cationic liposome envelope containing 17β-estradiol (E2) in its structure. Cationic liposomes protect cargo against the extracellular matrix, and E2 can increase its shuttling efficiency into cells. Using MCF-7 cells derived from estrogen receptor-positive ductal carcinoma, treatment with liposomes against ERα was found to decrease MCF-7 proliferation, and importantly the application of both the antisense against ERα and β exhibited an antiproliferative effect expressed as cell viability. Using qRT-PCR, it was shown that MT1A, NF-κB1 and K-ras genes, but not TFF1, were downregulated using E2-based liposomes (evaluated at P=0.05). Further indicators of oxidative stress were employed to assess the effect on treatment efficiency. Glutathione (GSH/GSSG redox ratio), metallothionein (MT) and malondialdehyde (MDA) confirmed a positive effect of antisense therapy resulting in their decreased levels in the MCF-7 cells. Based on these data, we suggest that E2-based liposomes offer sufficient transfer efficiency and moreover, due to the effect on NF-κB1, MT and GSH, tumor cells can be chemosensitized to increase treatment effectiveness.
Central European Institute of Technology Brno University of Technology CZ 616 00 Brno Czech Republic
Department of Chemistry and Biochemistry Mendel University in Brno CZ 613 00 Brno Czech Republic
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