Proteomic analysis of changes in the protein composition of MCF-7 human breast cancer cells induced by all-trans retinoic acid, 9-cis retinoic acid, and their combination
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
25455455
DOI
10.1016/j.toxlet.2014.09.030
PII: S0378-4274(14)01383-6
Knihovny.cz E-zdroje
- Klíčová slova
- Biomarker, Breast cancer, MCF-7, Proteomics, Retinoids,
- MeSH
- 2D gelová elektroforéza MeSH
- adaptorové proteiny vezikulární transportní metabolismus MeSH
- alitretinoin MeSH
- cytoskelet účinky léků metabolismus MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- invazivní růst nádoru MeSH
- jádro snRNP - proteiny metabolismus MeSH
- kofilin 1 metabolismus MeSH
- lidé MeSH
- nádorové proteiny metabolismus MeSH
- nádory prsu farmakoterapie metabolismus patologie MeSH
- pohyb buněk účinky léků MeSH
- proteiny tepelného šoku HSP27 metabolismus MeSH
- proteomika * metody MeSH
- protokoly protinádorové kombinované chemoterapie farmakologie MeSH
- tretinoin farmakologie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- adaptorové proteiny vezikulární transportní MeSH
- alitretinoin MeSH
- AP5B1 protein, human MeSH Prohlížeč
- CFL1 protein, human MeSH Prohlížeč
- jádro snRNP - proteiny MeSH
- kofilin 1 MeSH
- nádorové proteiny MeSH
- proteiny tepelného šoku HSP27 MeSH
- SNRPD3 protein, human MeSH Prohlížeč
- tretinoin MeSH
Retinoic acid (all-trans and 9-cis) isomers represent important therapeutic agents for many types of cancers, including human breast cancer. Changes in protein composition of the MCF-7 human breast cancer cells were induced by all-trans retinoic acid, 9-cis retinoic acid, and their combination and subsequently proteomic strategies based on bottom-up method were applied. Proposed approach was used for the analysis of proteins extracted from MCF-7 human breast cancer cell line utilizing a commercially manufactured kit RIPA and separated on two dimensional (2D) sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) after treatment with both retinoic acid isomers. We found significant differences in occurrence of proteins probably affecting the cell migration process in tumour cells. Heat shock protein 27, ribonucleoprotein SmD3, and cofilin-1 were significantly upregulated after treatment with combination of individual retinoic acid isomers. On the other hand, AP-5 complex subunit beta-1 shows the different response. Thus, the results might help to find the answer to important medical questions on (i) the identification of signaling pathways affected by retinoic acid isomers or (ii) how the observed proteomic pattern might reflect the effectiveness of retinoic acids treatment.
Institute of Analytical Chemistry of the ASCR v v i Brno Czech Republic
Institute of Experimental Endocrinology Slovak Academy of Sciences Bratislava Slovak Republic
Institute of Experimental Oncology Slovak Academy of Sciences Bratislava Slovak Republic
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