Retinoic acid (all-trans and 9-cis) isomers represent important therapeutic agents for many types of cancers, including human breast cancer. Changes in protein composition of the MCF-7 human breast cancer cells were induced by all-trans retinoic acid, 9-cis retinoic acid, and their combination and subsequently proteomic strategies based on bottom-up method were applied. Proposed approach was used for the analysis of proteins extracted from MCF-7 human breast cancer cell line utilizing a commercially manufactured kit RIPA and separated on two dimensional (2D) sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) after treatment with both retinoic acid isomers. We found significant differences in occurrence of proteins probably affecting the cell migration process in tumour cells. Heat shock protein 27, ribonucleoprotein SmD3, and cofilin-1 were significantly upregulated after treatment with combination of individual retinoic acid isomers. On the other hand, AP-5 complex subunit beta-1 shows the different response. Thus, the results might help to find the answer to important medical questions on (i) the identification of signaling pathways affected by retinoic acid isomers or (ii) how the observed proteomic pattern might reflect the effectiveness of retinoic acids treatment.
- MeSH
- 2D gelová elektroforéza MeSH
- adaptorové proteiny vezikulární transportní metabolismus MeSH
- cytoskelet účinky léků metabolismus MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- invazivní růst nádoru MeSH
- jádro snRNP - proteiny metabolismus MeSH
- kofilin 1 metabolismus MeSH
- lidé MeSH
- nádorové proteiny metabolismus MeSH
- nádory prsu farmakoterapie metabolismus patologie MeSH
- pohyb buněk účinky léků MeSH
- proteiny tepelného šoku HSP27 metabolismus MeSH
- proteomika * metody MeSH
- protokoly protinádorové kombinované chemoterapie farmakologie MeSH
- tretinoin farmakologie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
