Proteomic analysis of changes in the protein composition of MCF-7 human breast cancer cells induced by all-trans retinoic acid, 9-cis retinoic acid, and their combination
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
25455455
DOI
10.1016/j.toxlet.2014.09.030
PII: S0378-4274(14)01383-6
Knihovny.cz E-resources
- Keywords
- Biomarker, Breast cancer, MCF-7, Proteomics, Retinoids,
- MeSH
- Electrophoresis, Gel, Two-Dimensional MeSH
- Adaptor Proteins, Vesicular Transport metabolism MeSH
- Alitretinoin MeSH
- Cytoskeleton drug effects metabolism MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Neoplasm Invasiveness MeSH
- snRNP Core Proteins metabolism MeSH
- Cofilin 1 metabolism MeSH
- Humans MeSH
- Neoplasm Proteins metabolism MeSH
- Breast Neoplasms drug therapy metabolism pathology MeSH
- Cell Movement drug effects MeSH
- HSP27 Heat-Shock Proteins metabolism MeSH
- Proteomics * methods MeSH
- Antineoplastic Combined Chemotherapy Protocols pharmacology MeSH
- Tretinoin pharmacology MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Adaptor Proteins, Vesicular Transport MeSH
- Alitretinoin MeSH
- AP5B1 protein, human MeSH Browser
- CFL1 protein, human MeSH Browser
- snRNP Core Proteins MeSH
- Cofilin 1 MeSH
- Neoplasm Proteins MeSH
- HSP27 Heat-Shock Proteins MeSH
- SNRPD3 protein, human MeSH Browser
- Tretinoin MeSH
Retinoic acid (all-trans and 9-cis) isomers represent important therapeutic agents for many types of cancers, including human breast cancer. Changes in protein composition of the MCF-7 human breast cancer cells were induced by all-trans retinoic acid, 9-cis retinoic acid, and their combination and subsequently proteomic strategies based on bottom-up method were applied. Proposed approach was used for the analysis of proteins extracted from MCF-7 human breast cancer cell line utilizing a commercially manufactured kit RIPA and separated on two dimensional (2D) sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) after treatment with both retinoic acid isomers. We found significant differences in occurrence of proteins probably affecting the cell migration process in tumour cells. Heat shock protein 27, ribonucleoprotein SmD3, and cofilin-1 were significantly upregulated after treatment with combination of individual retinoic acid isomers. On the other hand, AP-5 complex subunit beta-1 shows the different response. Thus, the results might help to find the answer to important medical questions on (i) the identification of signaling pathways affected by retinoic acid isomers or (ii) how the observed proteomic pattern might reflect the effectiveness of retinoic acids treatment.
Institute of Analytical Chemistry of the ASCR v v i Brno Czech Republic
Institute of Experimental Endocrinology Slovak Academy of Sciences Bratislava Slovak Republic
Institute of Experimental Oncology Slovak Academy of Sciences Bratislava Slovak Republic
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