Differentially expressed proteins in human MCF-7 breast cancer cells sensitive and resistant to paclitaxel
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25557873
DOI
10.1016/j.yexcr.2014.12.005
PII: S0014-4827(14)00535-7
Knihovny.cz E-zdroje
- Klíčová slova
- 2-D electrophoresis, Breast cancer, Cathepsin D, Heat shock protein 27, TRIP6, Taxane resistance,
- MeSH
- adaptorové proteiny signální transdukční metabolismus MeSH
- ATPázy spojené s různými buněčnými aktivitami MeSH
- chemorezistence MeSH
- fytogenní protinádorové látky farmakologie MeSH
- kathepsin D metabolismus MeSH
- lidé MeSH
- MFC-7 buňky MeSH
- molekulární chaperony MeSH
- nádory prsu MeSH
- paclitaxel farmakologie MeSH
- proteasomový endopeptidasový komplex MeSH
- proteiny s doménou LIM metabolismus MeSH
- proteiny tepelného šoku HSP27 metabolismus MeSH
- proteiny teplotního šoku MeSH
- proteom metabolismus MeSH
- transkripční faktory metabolismus MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adaptorové proteiny signální transdukční MeSH
- ATPázy spojené s různými buněčnými aktivitami MeSH
- CTSD protein, human MeSH Prohlížeč
- fytogenní protinádorové látky MeSH
- HSPB1 protein, human MeSH Prohlížeč
- kathepsin D MeSH
- molekulární chaperony MeSH
- paclitaxel MeSH
- proteasomový endopeptidasový komplex MeSH
- proteiny s doménou LIM MeSH
- proteiny tepelného šoku HSP27 MeSH
- proteiny teplotního šoku MeSH
- proteom MeSH
- PSMC5 protein, human MeSH Prohlížeč
- transkripční faktory MeSH
Resistance of cancer cells to chemotherapeutic agents is one of the main causes of treatment failure. In order to detect proteins potentially involved in the mechanism of resistance to taxanes, we assessed differences in protein expression in MCF-7 breast cancer cells that are sensitive to paclitaxel and in the same cells with acquired resistance to paclitaxel (established in our lab). Proteins were separated using two-dimensional electrophoresis. Changes in their expression were determined and proteins with altered expression were identified using mass spectrometry. Changes in their expression were confirmed using western blot analysis. With these techniques, we found three proteins expressed differently in resistant MCF-7 cells, i.e., thyroid hormone-interacting protein 6 (TRIP6; upregulated to 650%), heat shock protein 27 (HSP27; downregulated to 50%) and cathepsin D (downregulated to 28%). Silencing of TRIP6 expression by specific siRNA leads to decreased number of grown resistant MCF-7 cells. In the present study we have pointed at some new directions in the studies of the mechanism of resistance to paclitaxel in breast cancer cells.
Citace poskytuje Crossref.org
The Role of TRIP6, ABCC3 and CPS1 Expression in Resistance of Ovarian Cancer to Taxanes
