Differentially expressed proteins in human MCF-7 breast cancer cells sensitive and resistant to paclitaxel
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25557873
DOI
10.1016/j.yexcr.2014.12.005
PII: S0014-4827(14)00535-7
Knihovny.cz E-resources
- Keywords
- 2-D electrophoresis, Breast cancer, Cathepsin D, Heat shock protein 27, TRIP6, Taxane resistance,
- MeSH
- Adaptor Proteins, Signal Transducing metabolism MeSH
- ATPases Associated with Diverse Cellular Activities MeSH
- Drug Resistance, Neoplasm MeSH
- Antineoplastic Agents, Phytogenic pharmacology MeSH
- Cathepsin D metabolism MeSH
- Humans MeSH
- MCF-7 Cells MeSH
- Molecular Chaperones MeSH
- Breast Neoplasms MeSH
- Paclitaxel pharmacology MeSH
- Proteasome Endopeptidase Complex MeSH
- LIM Domain Proteins metabolism MeSH
- HSP27 Heat-Shock Proteins metabolism MeSH
- Heat-Shock Proteins MeSH
- Proteome metabolism MeSH
- Transcription Factors metabolism MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adaptor Proteins, Signal Transducing MeSH
- ATPases Associated with Diverse Cellular Activities MeSH
- CTSD protein, human MeSH Browser
- Antineoplastic Agents, Phytogenic MeSH
- HSPB1 protein, human MeSH Browser
- Cathepsin D MeSH
- Molecular Chaperones MeSH
- Paclitaxel MeSH
- Proteasome Endopeptidase Complex MeSH
- LIM Domain Proteins MeSH
- HSP27 Heat-Shock Proteins MeSH
- Heat-Shock Proteins MeSH
- Proteome MeSH
- PSMC5 protein, human MeSH Browser
- Transcription Factors MeSH
Resistance of cancer cells to chemotherapeutic agents is one of the main causes of treatment failure. In order to detect proteins potentially involved in the mechanism of resistance to taxanes, we assessed differences in protein expression in MCF-7 breast cancer cells that are sensitive to paclitaxel and in the same cells with acquired resistance to paclitaxel (established in our lab). Proteins were separated using two-dimensional electrophoresis. Changes in their expression were determined and proteins with altered expression were identified using mass spectrometry. Changes in their expression were confirmed using western blot analysis. With these techniques, we found three proteins expressed differently in resistant MCF-7 cells, i.e., thyroid hormone-interacting protein 6 (TRIP6; upregulated to 650%), heat shock protein 27 (HSP27; downregulated to 50%) and cathepsin D (downregulated to 28%). Silencing of TRIP6 expression by specific siRNA leads to decreased number of grown resistant MCF-7 cells. In the present study we have pointed at some new directions in the studies of the mechanism of resistance to paclitaxel in breast cancer cells.
References provided by Crossref.org
The Role of TRIP6, ABCC3 and CPS1 Expression in Resistance of Ovarian Cancer to Taxanes
