Common scab of potatoes is a disease, which is difficult to manage due to complex interactions of the pathogenic bacteria (Streptomyces spp.) with soil, microbial community and potato plants. In Bohemian-Moravian Highlands in the Czech Republic two sites (Vyklantice and Zdirec) were selected for a study of common scab disease suppressivity. At both sites, a field with low disease severity occurs next to one with high severity and the situation was regularly observed over four decades although all four fields undergo a crop rotation. In the four fields, quantities of bacteria, actinobacteria and the gene txtB from the biosynthetic gene cluster of thaxtomin, the main pathogenicity factor of common scab, were analyzed by real-time PCR. Microbial community structure was compared by terminal fragment length polymorphism analysis. Soil and potato periderm were characterized by contents of carbon, nitrogen, phosphorus, sulphur, calcium, magnesium, and iron. Quality of organic matter was assessed by high performance liquid chromatography of soil extracts. The study demonstrated that the suppressive character of the fields is locally specific. At Zdirec, the suppressivity was associated with low txtB gene copies in bulk soil, while at Vyklantice site it was associated with low txtB gene copies in the tuberosphere. The differences were discussed with respect to the effect of abiotic conditions at Zdirec and interaction between potato plant and soil microbial community at Vyklantice. Soil pH, Ca soil content or cation concentrations, although different were not in the range to predict the disease severity. Low severity of common scab was associated with low content of soil C, N, C/N, Ca and Fe suggesting that oligotrophic conditions may be favorable to common scab suppression.

Biology Centre of the Academy of Sciences of the Czech Republic v v i Institute of Soil Biology Ceske Budejovice Czech Republic

Crop Research Institute Dept Epidemiology and Ecology of Microorganisms Prague Czech Republic

Faculty of Agriculture Dept Plant Production University of South Bohemia Ceske Budejovice Czech Republic

Faculty of Mathematics and Physics Dept Probability and Mathematical Statistics Charles University Prague Czech Republic

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Dees MW, Wanner LA (2012) In Search of Better Management of Potato Common Scab. Potato Res 55: 249–268. 10.1007/s11540-012-9206-9 DOI

Archuleta JG, Easton GD (1981) The cause of deep-pitted scab of potatoes. Am Potato J 58: 385–392. 10.1007/BF02852950 DOI

Kers JA, Cameron KD, Joshi MV, Bukhalid RA, Morello JE, et al. (2005) A large, mobile pathogenicity island confers plant pathogenicity on Streptomyces species. Mol Microbiol 55: 1025–1033. 10.1111/j.1365-2958.2004.04461.x PubMed DOI

Hiltunen LH, Laakso I, Chobot V, Hakala K, Weckman A, Valkonen JPT (2006) The influence of thaxtomins in different combinations and concentrations on growth of micropropagated potato shoot cultures. J Agric Food Chem 54: 3372–3379. 10.1021/jf060270m PubMed DOI

Lazarovits G, Hill J, Patterson G, Kenneth L, Conn KL, Crump NS (2007) Edaphic soil levels of mineral nutrients, pH, organic matter, and cationic exchange capacity in the geocaulosphere associated with potato common scab. Phytopathology 97: 1071–1082. 10.1094/PHYTO-97-9-1071 PubMed DOI

Rosenzweig N, Tiedje JM, Quensen JF III, Meng Q, Hao JJ (2012) Microbial communities associated with potato common scab-suppressive soil determined by pyrosequencing analyses. Plant Dis 96: 718–725. 10.1094/PDIS-07-11-0571 PubMed DOI

Baker KF, Cook RJ (1974) Biological control of plant pathogens. San Francisco: WH Freeman and Company; 10.2307/3758248 DOI

Meng QX, Yin JF, Rosenzweig N, Douches D, Hao JJ (2012) Culture-based assessment of microbial communities in soil suppressive to potato common scab. Plant Dis 96: 712–717. 10.1094/PDIS-05-11-0441 PubMed DOI

Almario J, Kyselková M, Kopecký J, Ságová-Marečková M, Muller D, et al. (2013) Assessment of the relationship between geologic origin of soil, rhizobacterial community composition and soil receptivity to tobacco black root rot in Savoie region (France). Plant Soil 371: 397–408. 10.1007/s11104-013-1677-1 DOI

Wenzl H, Demel J (1967) Bildskalen für die Beurteilung von Kartoffelschorf und Rhizoctonia-Pocken. Der Pflanzenarzt 20: 77–78.

Kopáček J, Hejzlar J (1995) Semi-micro determination of total phosphorus in soils, sediments, and organic materials: A simplified perchloric acid digestion procedure. Commun Soil Sci Plant Anal 26: 1935–1946. 10.1080/00103629509369419 DOI

Ságová-Marečková M, Čermák L, Novotná J, Plháčková K, Forstová J, et al. (2008) Innovative methods for soil DNA purification tested in soils of widely differing characteristics. Appl Environ Microbiol 74: 2902–2907. 10.1128/AEM.02161-07 PubMed DOI PMC

Cermak L, Kopecky J, Novotna J, Omelka M, Parkhomenko N, et al. (2008) Bacterial communities of two contrasting soils reacted differently to lincomycin treatment. Appl Soil Ecol 40: 348–358. 10.1016/j.apsoil.2008.06.001 DOI

Kyselková M, Kopecký J, Felföldi T, Čermák L, Omelka M, et al. (2008) Development of a 16S rRNA gene-based prototype microarray for the detection of selected actinomycetes genera. Antonie Van Leeuwenhoek 94: 439–453. 10.1007/s10482-008-9261-z PubMed DOI

Lane D (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M, editors. Nucleic acid techniques in bacterial systematics. West Sussex: John Wiley & Sons; pp. 115–175.

Muyzer G, de Waal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59: 695–700. PubMed PMC

Stach JEM, Maldonado LA, Ward AC, Goodfellow M, Bull AT (2003) New primers for the class Actinobacteria: application to marine and terrestrial environments. Environ Microbiol 5: 828–841. 10.1046/j.1462-2920.2003.00483.x PubMed DOI

Qu X, Wanner LA, Christ BJ (2008) Using the TxtAB Operon to Quantify Pathogenic Streptomyces in Potato Tubers and Soil. Phytopathology 98: 405–412. 10.1094/PHYTO-98-4-0405 PubMed DOI

Venables WN, Ripley BD (2002) Modern applied statistics with S. New York: Springer; 10.1007/978-0-387-21706-2 DOI

McArdle BH, Anderson MJ (2001) Fitting multivariate models to community data: A comment on distance based redundancy analysis. Ecology 82: 290–297. 10.1890/0012-9658(2001)082[0290:FMMTCD]2.0.CO;2 DOI

Gijbels I, Omelka M (2013) Testing for Homogeneity of Multivariate Dispersions Using Dissimilarity Measures. Biometrics 69: 137–145. 10.1111/j.1541-0420.2012.01797.x PubMed DOI

Omelka M, Pauly M (2012) Testing equality of correlation coefficients in two populations via permutation methods. J Stat Plan Infer 142: 1396–1406. 10.1016/j.jspi.2011.12.018 DOI

R Core Team (2014) R: A Language and Environment for Statistical Computing. Vienna: R Foundation for Statistical Computing.

Kyselkova M, Moenne-Loccoz Y (2012) Pseudomonas and other Microbes in Disease-Suppressive Soils. In: Lichtfouse E, editor. Organic Fertilisation, Soil Quality and Human Health 93. Sustainable Agriculture Reviews 9. Springer Science+Business Media B.V. pp. 93–140. 10.1007/978-94-007-4113-3_5 DOI

Kinkel LL, Bakker MG, Schlatter DC (2011) A Coevolutionary Framework for Managing Disease-Suppressive Soils. Annu Rev Phytopathol 49: 47–67. 10.1146/annurev-phyto-072910-095232 PubMed DOI

Lambert DH, Powelson ML, Stevenson WR (2005) Nutritional interactions influencing diseases of potato. Am J Potato Res 82: 309–319. 10.1007/BF02871961 DOI

Davis JR, McDole RE, Callihan RH (1976) Fertilizer effects on common scab of potato and the relation of calcium and phosphatephosphorus. Phytopathology 66: 1236–1241. 10.1094/Phyto-66-1236 DOI

Lacey MJ, Wilson CR (2001) Relationship of common scab incidence of potatoes grown in Tasmanian ferrosol soils with pH, exchangeable cations and other chemical properties of those soils. J Phytopathol 149: 679–683. 10.1046/j.1439-0434.2001.00693.x DOI

Errakhi R, Dauphin A, Meimoun P, Lehner A, Reboutier D, et al. (2008) An early Ca2+ influx is a prerequisite to thaxtomin A-induced cell death in Arabidopsis thaliana cells. J Exp Bot 59: 4259–4270. 10.1093/jxb/ern267 PubMed DOI

Merchant SS (2010) The Elements of Plant Micronutrients. Plant Physiology 154: 512–515. 10.1104/pp.110.161810 PubMed DOI PMC

Bailey K, Lazarovits G (2003) Suppressing soil-borne diseases with residue management and organic amendments. Soil Tillage Res 72: 169–180. 10.1016/S0167-1987(03)00086-2 DOI

Bonanomi G, Antignani V, Pane C, Scala F (2007) Suppression of soilborne fungal diseases with organic amendments. J Plant Pathol 89: 311–324.

Noble R, Coventry E (2005) Suppression of soil-borne plant diseases with composts: a review. Biocontrol Sci Tech 15: 3–20. 10.1080/09583150400015904 DOI

Kyselková M, Almario J, Kopecký J, Ságová-Marečková M, Haurat J, et al. (2014) Evaluation of rhizobacterial indicators of tobacco black root rot suppressiveness in farmers’ fields. Environ Microbiol Rep 6: 346–353. 10.1111/1758-2229.12131 PubMed DOI

Yergeau E, Bezemer TM, Hedlund K, Mortimer SR, Kowalchuk GA, et al. (2010) Influences of space, soil, nematodes and plants on microbial community composition of chalk grassland soils. Environ Microbiol 12: 2096–2106. 10.1111/j.1462-2920.2009.02053.x PubMed DOI

Schlatter DC, DavelosBaines AL, Xiao K, Kinkel LL (2013) Resource use of soilborne Streptomyces varies with location, phylogeny, and nitrogen amendment. Microb Ecol 66: 961–971. 10.1007/s00248-013-0280-6 PubMed DOI

Weinert N, Meincke R, Gottwald C, Heuer H, Schloter M, et al. (2010) Bacterial diversity on the surface of potato tubers in soil and the in£uence of the plant genotype. FEMS Microbiol Ecol 74: 114–123. 10.1111/j.1574-6941.2010.00936.x PubMed DOI

Mazzola M (2002) Mechanisms of natural soil suppressiveness to soilborne diseases. Anton Leeuw 81:557–564. 10.1023/A:1020557523557 PubMed DOI

Meera MS, Shivanna MB, Kageyama K, Hyakumachi M (1995) Responses of cucumber cultivars to induction of systemic resistance against anthracnose by plant growth promoting fungi. Eur J Plant Pathol 101:421–430. 10.1007/BF01874856 DOI

Davis JR, McDole RE, Callihan RH (1976) Fertilizer Effects on common scab of potato and the relation of calcium and phosphate-phosphorus. Phytopathology 66: 1404–1410. 10.1094/Phyto-66-1236 DOI

Kristufek V, Divis J, Dostalkova I, Kalcik J (2000) Accumulation of mineral elements in tuber periderm of potato cultivars differing in susceptibility to common scab. Potato Res 43: 107–114. 10.1007/BF02357951 DOI

Tokala RK, Strap JL, Jung CM, Crawford DL, Salove MH, et al. (2002) Novel plant-microbe rhizosphere interaction involving Streptomyces lydicus WYEC108 and the pea plant (Pisum sativum). Appl Environ Microbiol 68: 2161–2171. 10.1128/AEM.68.5.2161-2171.2002 PubMed DOI PMC

Lorang JM, Anderson NA, Lauer FI, Wildung DK (1989) Disease decline in a Minnesota potato scab plot. Am Potato J 66: 531.

Bacterial, archaeal and micro-eukaryotic communities characterize a disease-suppressive or conducive soil and a cultivar resistant or susceptible to common scab

A Short-Term Response of Soil Microbial Communities to Cadmium and Organic Substrate Amendment in Long-Term Contaminated Soil by Toxic Elements

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