Cholesterol under oxidative stress-How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25795515
DOI
10.1016/j.freeradbiomed.2015.03.006
PII: S0891-5849(15)00118-5
Knihovny.cz E-resources
- Keywords
- DPH anisotropy, Laurdan fluorescence, Liposomes, Molecular dynamics simulations, NMR measurements, Oxysterols, Phospholipid bilayers, Tilt modulus,
- MeSH
- Cell Membrane chemistry MeSH
- Fluorescence Polarization MeSH
- Phosphatidylcholines chemistry MeSH
- Hydroxycholesterols chemistry MeSH
- Lipid Bilayers chemistry MeSH
- Oxidative Stress * MeSH
- Molecular Dynamics Simulation MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1-palmitoyl-2-oleoylphosphatidylcholine MeSH Browser
- Phosphatidylcholines MeSH
- Hydroxycholesterols MeSH
- Lipid Bilayers MeSH
The behavior of oxysterols in phospholipid membranes and their effects on membrane properties were investigated by means of dynamic light scattering, fluorescence spectroscopy, NMR, and extensive atomistic simulations. Two families of oxysterols were scrutinized-tail-oxidized sterols, which are mostly produced by enzymatic processes, and ring-oxidized sterols, formed mostly via reactions with free radicals. The former family of sterols was found to behave similar to cholesterol in terms of molecular orientation, roughly parallel to the bilayer normal, leading to increasing membrane stiffness and suppression of its membrane permeability. In contrast, ring-oxidized sterols behave quantitatively differently from cholesterol. They acquire tilted orientations and therefore disrupt the bilayer structure with potential implications for signaling and other biochemical processes in the membranes.
Department of Physics and Chemistry University of Oulu P O Box 3000 FI 90014 Oulu Finland
Department of Physics Tampere University of Technology P O Box 692 FI 33101 Tampere Finland
References provided by Crossref.org
The Two Faces of the Liquid Ordered Phase
What Does Time-Dependent Fluorescence Shift (TDFS) in Biomembranes (and Proteins) Report on?
Tail-Oxidized Cholesterol Enhances Membrane Permeability for Small Solutes
