Cholesterol under oxidative stress-How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25795515
DOI
10.1016/j.freeradbiomed.2015.03.006
PII: S0891-5849(15)00118-5
Knihovny.cz E-zdroje
- Klíčová slova
- DPH anisotropy, Laurdan fluorescence, Liposomes, Molecular dynamics simulations, NMR measurements, Oxysterols, Phospholipid bilayers, Tilt modulus,
- MeSH
- buněčná membrána chemie MeSH
- fluorescenční polarizace MeSH
- fosfatidylcholiny chemie MeSH
- hydroxycholesteroly chemie MeSH
- lipidové dvojvrstvy chemie MeSH
- oxidační stres * MeSH
- simulace molekulární dynamiky MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 1-palmitoyl-2-oleoylphosphatidylcholine MeSH Prohlížeč
- fosfatidylcholiny MeSH
- hydroxycholesteroly MeSH
- lipidové dvojvrstvy MeSH
The behavior of oxysterols in phospholipid membranes and their effects on membrane properties were investigated by means of dynamic light scattering, fluorescence spectroscopy, NMR, and extensive atomistic simulations. Two families of oxysterols were scrutinized-tail-oxidized sterols, which are mostly produced by enzymatic processes, and ring-oxidized sterols, formed mostly via reactions with free radicals. The former family of sterols was found to behave similar to cholesterol in terms of molecular orientation, roughly parallel to the bilayer normal, leading to increasing membrane stiffness and suppression of its membrane permeability. In contrast, ring-oxidized sterols behave quantitatively differently from cholesterol. They acquire tilted orientations and therefore disrupt the bilayer structure with potential implications for signaling and other biochemical processes in the membranes.
Department of Physics and Chemistry University of Oulu P O Box 3000 FI 90014 Oulu Finland
Department of Physics Tampere University of Technology P O Box 692 FI 33101 Tampere Finland
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