In pigs, in vitro production is difficult with a high occurrence of polyspermy and low blastocyst formation rates. To test the hypothesis that this may, at least in part, be due to chromosomal errors, we employed whole genome amplification and comparative genomic hybridization, performing comprehensive chromosome analysis to assess both cells of the two-cell stage in vitro porcine embryos. We thus described the incidence, nature and origin of chromosome abnormalities, i.e. whether they derived from incorrect meiotic division during gametogenesis or aberrant mitotic division in the zygote. We observed that 19 out of 51 (37%) of two-cell stage early pig IVP embryos had a chromosome abnormality, mostly originating from an abnormal division in the zygote. Moreover, we frequently encountered multiple aneuploidies and segmental chromosome aberrations. These results indicate that the pig may be particularly sensitive to in vitro production, which may, in turn, be due to incorrect chromosome segregations during meiosis and early cleavage divisions. We thus accept our hypothesis that chromosome abnormality could explain poor IVP outcomes in pigs.

Central European Institute of Technology Veterinary Research Institute Hudcova 70 Brno 621 00 Czech Republic

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Hum Reprod Update. 2002 Jul-Aug;8(4):333-43 PubMed

Theriogenology. 2014 Jan 1;81(1):24-37 PubMed

Cytogenet Genome Res. 2009;126(1-2):210-6 PubMed

Hum Reprod. 1997 Apr;12(4):780-4 PubMed

Mol Reprod Dev. 2005 Sep;72(1):77-87 PubMed

Anim Reprod Sci. 2008 Aug;107(1-2):131-47 PubMed

Hum Reprod. 2002 Feb;17(2):413-9 PubMed

Eur J Obstet Gynecol Reprod Biol. 2014 Apr;175:152-6 PubMed

Hum Reprod Update. 2011 Sep-Oct;17(5):620-7 PubMed

Biol Reprod. 1994 Sep;51(3):373-9 PubMed

Hum Reprod. 2013 Jan;28(1):256-64 PubMed

Prenat Diagn. 2000 Jul;20(7):552-60 PubMed

Theriogenology. 2004 Jan 15;61(2-3):551-60 PubMed

Chromosome Res. 2007;15(3):399-408 PubMed

J Reprod Fertil. 1997 Sep;111(1):101-8 PubMed

Cytogenet Genome Res. 2003;102(1-4):179-83 PubMed

PLoS One. 2011 Apr 27;6(4):e18892 PubMed

Chromosome Res. 2012 May;20(4):447-60 PubMed

Biol Reprod. 1991 Jan;44(1):62-8 PubMed

Reprod Biomed Online. 2004 Jun;8(6):701-11 PubMed

Biol Reprod. 1999 Jun;60(6):1273-8 PubMed

Mol Reprod Dev. 1996 Jul;44(3):417-22 PubMed

Cancer Genet Cytogenet. 2000 Oct 1;122(1):7-12 PubMed

Theriogenology. 2007 Sep 15;68(5):755-62 PubMed

Hum Reprod. 2006 Jan;21(1):223-33 PubMed

Nat Commun. 2012;3:1251 PubMed

Nature. 1988 Mar 31;332(6163):459-61 PubMed

Reprod Biomed Online. 2008 Dec;17(6):841-7 PubMed

Biol Reprod. 1999 Nov;61(5):1340-6 PubMed

Theriogenology. 2003 Nov;60(8):1569-80 PubMed

Prenat Diagn. 1998 Mar;18(3):201-6 PubMed

PLoS One. 2012;7(1):e30335 PubMed

Hum Mol Genet. 2007 Oct 15;16 Spec No. 2:R203-8 PubMed

Nat Med. 2009 May;15(5):577-83 PubMed

Hum Reprod. 1994 Jul;9(7):1265-70 PubMed

Cytogenet Genome Res. 2011;133(2-4):141-8 PubMed

Hum Reprod. 2004 Dec;19(12):2859-68 PubMed

J Reprod Dev. 2008 Feb;54(1):22-9 PubMed

Theriogenology. 2003 Aug;60(3):571-81 PubMed

Fertil Steril. 2003 Oct;80(4):860-8 PubMed