Data on the frequency of aneuploidy in farm animals are lacking and there is the need for a reliable technique which is capable of detecting all chromosomes simultaneously in a single cell. With the employment of comparative genomic hybridization coupled with the whole genome amplification technique, this study brings new information regarding the aneuploidy of individual chromosomes in pigs. Focus is directed on in vivo porcine blastocysts and late morulas, 4.7% of which were found to carry chromosomal abnormality. Further, ploidy abnormalities were examined using FISH in a sample of porcine embryos. True polyploidy was relatively rare (1.6%), whilst mixoploidy was presented in 46.8% of embryos, however it was restricted to only a small number of cells per embryo. The combined data indicates that aneuploidy is not a prevalent cause of embryo mortality in pigs.

Veterinary Research Institute Brno Czech Republic

Zobrazit více v PubMed

King WA. Chromosome variation in the embryos of domestic animals. Cytogenet Genome Res. 2008;120:81–90. PubMed

Pauciullo A, Nicodemo D, Castello A, Cosenza G, Ramunno L, et al. Chromosomal unbalancements in sperm and oocytes of two Italian cattle breeds as determined by dual color fluorescent in situ hybridization (FISH). Ital J Anim Sci. 2009;8:123–125.

Coppola G, Alexander B, Di Berardino D, St John E, Basrur PK, et al. Use of cross-species in-situ hybridization (ZOO-FISH) to assess chromosome abnormalities in day-6 in-vivo- or in-vitro-produced sheep embryos. Chromosome Res. 2007;15:399–408. PubMed

Rambags BPB, Krijtenburg PJ, Van Drie HF, Lazzari G, Galli C, et al. Numerical chromosomal abnormalities in equine embryos produced in vivo and in vitro. Mol Reprod Dev. 2005;72:77–87. PubMed

Zuccotti M, Boiani M, Garagna S, Redi CA. Analysis of aneuploidy rate in antral and ovulated mouse oocytes during female aging. Mol Reprod Dev. 1998;50:305–312. PubMed

Ducos A, Berland HM, Pinton A, Guillemot E, Seguela A, et al. Nine new cases of reciprocal translocation in the domestic pig (Sus scrofa domestica L.). J Hered. 1998;89:136–142. PubMed

Hornak M, Jeseta M, Musilova P, Pavlok A, Kubelka M, et al. Frequency of aneuploidy related to age in porcine oocytes. PLoS ONE. 2011;6(4):e18892. PubMed PMC

Hornak M, Hulinska P, Musilova P, Kubickova S, Rubes J. Investigation of chromosome aneuploidies in early porcine embryos using comparative genomic hybridization. Cytogenet Genome Res. 2009;126:210–216. PubMed

Gutierrez-Mateo C, Colls P, Sanchez-Garcia J, Escudero T, Prates R, et al. Validation of microarray comparative genomic hybridization for comprehensive chromosome analysis of embryos. Fertil Steril. 2011;95:953–958. PubMed

Keskintepe L, Sher G, Keskintepe M. Reproductive oocyte/embryo genetic analysis: comparison between fluorescence in-situ hybridization and comparative genomic hybridization. Reprod Biomed Online. 2007;15:303–309. PubMed

Gabriel AS, Thornhill AR, Ottolini CS, Gordon A, Brown APC, et al. Array comparative genomic hybridisation on first polar bodies suggests that non-disjunction is not the predominant mechanism leading to aneuploidy in humans. J Med Genet. 2011;48:433–437. PubMed

Lechniak D, Warzych E, Pers-Kamczyc E, Sosnowski J, Antosik P, et al. Gilts and sows produce similar rate of diploid oocytes in vitro whereas the incidence of aneuploidy differs significantly. Theriogenology. 2007;68:755–762. PubMed

Zudova D, Rezacova O, Kubickova S, Rubes J. Aneuploidy detection in porcine embryos using fluorescence in situ hybridization. Cytogenet Genome Res. 2003;102:179–183. PubMed

Vozdova M, Machatkova M, Kubickova S, Zudova D, Jokesova E, et al. Frequency of aneuploidy in pig oocytes matured in vitro and of the corresponding first polar bodies detected by fluorescent in situ hybridization. Theriogenology. 2001;56:771–776. PubMed

Rubes J, Vozdova M, Kubickova S. Aneuploidy in pig sperm: multicolor fluorescence in situ hybridization using probes for chromosomes 1, 10, and Y. Cytogenet Cell Genet. 1999;85:200–204. PubMed

Sosnowski J, Waroczyk M, Switonski M. Chromosome abnormalities in secondary pig oocytes matured in vitro. Theriogenology. 2003;60:571–581. PubMed

Frumkin T, Malcov M, Yaron Y, Ben-Yosef D. Elucidating the origin of chromosomal aberrations in IVF embryos by preimplantation genetic analysis. Mol Cell Endocrinol. 2008;282:112–119. PubMed

Johnson DS, Cinnioglu C, Ross R, Filby A, Gemelos G, et al. Comprehensive analysis of karyotypic mosaicism between trophectoderm and inner cell mass. Mol Hum Reprod. 2010;16:944–949. PubMed PMC

Vanneste E, Voet T, Le Caignec C, Ampe M, Konings P, et al. Chromosome instability is common in human cleavage-stage embryos. Nat Med. 2009;15:577–583. PubMed

McCauley TC, Mazza MR, Didion BA, Mao J, Wu G, et al. Chromosomal abnormalities in day-6, in vitro-produced pig embryos. Theriogenology. 2003;60:1569–1580. PubMed

Zijlstra C, Kidson A, Schoevers EJ, Daemen AJJM, Tharasanit T, et al. Blastocyst morphology, actin cytoskeleton quality and chromosome content are correlated with embryo quality in the pig. Theriogenology. 2008;70:923–935. PubMed

Dolch KM, Chrisman CL. Cytogenetic Analysis of Pre-Implantation Blastocysts from Prepuberal Gilts Treated with Gonadotropins. Am J Vet Res. 1981;42:344–346. PubMed

Vanderhoeven FA, Cuijpers MP, Deboer P. Karyotypes of 3-Day-Old Or 4-Day-Old Pig Embryos After Short Invitro Culture. J Reprod Fertil. 1985;75:593–597. PubMed

Long SE, Williams CV. A Comparison of the Chromosome Complement of Inner Cell Mass and Trophoblast Cells in Day-10 Pig Embryos. J Reprod Fertil. 1982;66:645–648. PubMed

McFeely RA. Chromosome Abnormalities in Early Embryos of Pig. J Reprod Fertil. 1967;13:579–581. PubMed

Moon RG, Rashad MN, Mi MP. Example of Polyploidy in Pig Blastocysts. J Reprod Fertil. 1975;45:147–149. PubMed

Coonen E, Dumoulin JCM, Ramaekers FCS, Hopman AHN. Optimal Preparation of Preimplantation Embryo Interphase Nuclei for Analysis by Fluorescence In-Situ Hybridization. Human Reprod. 1994;9:533–537. PubMed

Yerle M, Goureau A, Gellin J, Le Tissier P, Moran C. Rapid mapping of cosmid clones on pig chromosomes by fluorescence in situ hybridization. Mamm Genome. 1994;5:34–37. PubMed

Koenig JLF, Stormshak F. Cytogenetic Evaluation of Ova from Pubertal and 3rd-Estrous Gilts. Biol Reprod. 1993;49:1158–1162. PubMed

Ulloa CMU, Yoshizawa M, Komoriya E, Mitsui A, Nagai T, et al. The blastocyst production rate and incidence of chromosomal abnormalities by developmental stage in in vitro produced porcine embryos. J Reprod Develop. 2008;54:22–29. PubMed

Early onset of APC/C activity renders SAC inefficient in mouse embryos

Chromosome Division in Early Embryos-Is Everything under Control? And Is the Cell Size Important?

A high incidence of chromosome abnormalities in two-cell stage porcine IVP embryos