Proton-induced direct and indirect damage of plasmid DNA
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- biologické modely MeSH
- dvouřetězcové zlomy DNA MeSH
- elektroforéza v agarovém gelu MeSH
- enzymy opravy DNA metabolismus MeSH
- lineární přenos energie MeSH
- plazmidy metabolismus účinky záření MeSH
- poškození DNA * MeSH
- protony škodlivé účinky MeSH
- roztoky MeSH
- vztah dávky záření a odpovědi MeSH
- záření gama škodlivé účinky MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- enzymy opravy DNA MeSH
- protony MeSH
- roztoky MeSH
Clustered DNA damage induced by 10, 20 and 30 MeV protons in pBR322 plasmid DNA was investigated. Besides determination of strand breaks, additional lesions were detected using base excision repair enzymes. The plasmid was irradiated in dry form, where indirect radiation effects were almost fully suppressed, and in water solution containing only minimal residual radical scavenger. Simultaneous irradiation of the plasmid DNA in the dry form and in the solution demonstrated the contribution of the indirect effect as prevalent. The damage composition slightly differed when comparing the results for liquid and dry samples. The obtained data were also subjected to analysis concerning different methodological approaches, particularly the influence of irradiation geometry, models used for calculation of strand break yields and interpretation of the strand breaks detected with the enzymes. It was shown that these parameters strongly affect the results.
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