Gene expression profiling of changes induced by maternal diabetes in the embryonic heart
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26073000
DOI
10.1016/j.reprotox.2015.06.045
PII: S0890-6238(15)00154-9
Knihovny.cz E-resources
- Keywords
- Cardiovascular defects, Diabetic embryopathy, Gene expression, Vascular endothelial growth factor A (VEGF-A),
- MeSH
- Embryo, Mammalian MeSH
- Diabetes Mellitus, Experimental genetics metabolism MeSH
- Hypoxia-Inducible Factor 1, alpha Subunit metabolism MeSH
- Hypoxia genetics metabolism MeSH
- Mice, Inbred Strains MeSH
- RNA, Messenger metabolism MeSH
- Myocardium metabolism MeSH
- Heart embryology MeSH
- Gene Expression Profiling MeSH
- Pregnancy in Diabetics genetics metabolism MeSH
- Pregnancy MeSH
- Vascular Endothelial Growth Factor A genetics MeSH
- Gene Expression Regulation, Developmental * MeSH
- Animals MeSH
- Check Tag
- Pregnancy MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Hypoxia-Inducible Factor 1, alpha Subunit MeSH
- Hif1a protein, mouse MeSH Browser
- RNA, Messenger MeSH
- vascular endothelial growth factor A, mouse MeSH Browser
- Vascular Endothelial Growth Factor A MeSH
Cardiovascular defects are one of the most common congenital defects associated with maternal diabetes. Based on whole embryo gene expression microarray analysis, 11 genes were chosen for temporal expression analysis of diabetes-exposed hearts. The majority of the selected genes were deregulated in diabetes-exposed hearts compared to our controls at E13.5, E14.5, and E18.5. We showed increased hypoxia and HIF-1α protein levels in diabetes-exposed hearts at E10.5, which is a critical time point for the induction of developmental defects associated with diabetic embryopathy. Additionally, we found increased cardiac Vegfa levels that might trigger developmental abnormalities associated with diabetic embryopathy. Our results show that maternal diabetes affects the temporal expression pattern of gene encoding molecules involved in heart development and tissue remodelling and that these molecules might affect heart maturation processes and thus, the final outcome of diabetic pregnancies.
References provided by Crossref.org
HIF-1, Metabolism, and Diabetes in the Embryonic and Adult Heart
