Diverse effects of naturally occurring base lesions on the structure and stability of the human telomere DNA quadruplex
Jazyk angličtina Země Francie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem, přehledy
PubMed
26188111
DOI
10.1016/j.biochi.2015.07.013
PII: S0300-9084(15)00221-7
Knihovny.cz E-zdroje
- Klíčová slova
- Base modification, Circular dichroism spectroscopy, DNA secondary structure, Guanine quadruplex, Oxidative damage,
- MeSH
- cirkulární dichroismus MeSH
- G-kvadruplexy * MeSH
- konformace nukleové kyseliny * MeSH
- lidé MeSH
- molekulární modely * MeSH
- poškození DNA genetika MeSH
- telomery chemie genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
Various base lesions continuously form in cellular nucleic acids and the unrepaired lesions are promutagenic and procarcinogenic. Though natural base lesions have been extensively studied in double-stranded DNA models, these studies are only less than a decade old for non-canonical DNA models, such as quadruplexes. Here we present a report on the effects of three frequently occurring natural lesions that can form in the TTA loops on the structure of the human telomere quadruplex d[AG3(TTAG3)3]. We compared the effect of the abasic site and 8-oxoadenine replacing adenine and 5-hydroxymethyluracil substituting for thymine. The results showed that the three lesions impacted the stability and quadruplex folding in markedly different ways. The effects depended on the type of lesion and the position in the sequence. Analogous lesions of guanine in the G-tetrads extensively destabilized the quadruplex and the effects depended more on the position than on the type of lesion. The distinct effects of the loop substitutions as well as comparison of the modifications of the loops and the quadruplex tetrads are discussed in this communication.
Citace poskytuje Crossref.org
Diversity of Parallel Guanine Quadruplexes Induced by Guanine Substitutions
i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions