Effects of the non-steroidal anti-inflammatory drug(NSAID) naproxen on gene expression of antioxidant enzymes in zebrafish (Danio rerio)
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26233559
DOI
10.1016/j.etap.2015.07.009
PII: S1382-6689(15)30035-1
Knihovny.cz E-resources
- Keywords
- Fish, Naproxen, Oxidative stress, mRNA expression,
- MeSH
- Anti-Inflammatory Agents, Non-Steroidal adverse effects MeSH
- Antioxidants metabolism MeSH
- Zebrafish embryology genetics MeSH
- Glutathione Transferase genetics MeSH
- Ion Channels genetics MeSH
- Liver enzymology MeSH
- Catalase genetics MeSH
- Mitochondrial Proteins genetics MeSH
- Naproxen adverse effects MeSH
- Zebrafish Proteins genetics MeSH
- Gene Expression Regulation, Enzymologic drug effects MeSH
- Intestines enzymology MeSH
- Uncoupling Protein 2 MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anti-Inflammatory Agents, Non-Steroidal MeSH
- Antioxidants MeSH
- Glutathione Transferase MeSH
- Ion Channels MeSH
- Catalase MeSH
- Mitochondrial Proteins MeSH
- Naproxen MeSH
- Zebrafish Proteins MeSH
- UCP2 protein, human MeSH Browser
- Uncoupling Protein 2 MeSH
The aim of this study was to investigate the effects of naproxen on the gene expression of antioxidant enzymes in adult zebrafish. Surprisingly, after 2 weeks exposure no significant effect on the mRNA expression of the target genes was found in the liver. However, mRNA levels of three genes were altered significantly in the intestine. The expression of Ucp-2 decreased at the environmental concentration of 1μg/L while mRNA expression of GST p2 increased at the concentration of 100μg/L. The mRNA level for the antioxidant enzyme CAT was up-regulated significantly at both the concentrations used. Exposure to naproxen caused only moderate effects on the expression of antioxidant genes in the intestine rather than in the liver, which demonstrates that the intestine is more sensitive to waterborne naproxen exposure than the liver. Interestingly, the adverse side effects of NSAIDs occur in the gastrointestinal tract of humans. To our knowledge, this is the first study that has focused on transcriptional effects of naproxen on zebrafish.
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