Identification of STAT3 and STAT5 proteins in the rat suprachiasmatic nucleus and the Day/Night difference in astrocytic STAT3 phosphorylation in response to lipopolysaccharide
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26420542
DOI
10.1002/jnr.23673
Knihovny.cz E-resources
- Keywords
- STAT proteins, circadian clock, lipopolysaccharide, rat, suprachiasmatic nucleus,
- MeSH
- Analysis of Variance MeSH
- Time Factors MeSH
- Circadian Rhythm drug effects MeSH
- Phosphorylation drug effects MeSH
- Glial Fibrillary Acidic Protein metabolism MeSH
- Rats MeSH
- Lipopolysaccharides pharmacology MeSH
- Suprachiasmatic Nucleus cytology drug effects MeSH
- Rats, Wistar MeSH
- Gene Expression Regulation drug effects MeSH
- STAT3 Transcription Factor metabolism MeSH
- STAT5 Transcription Factor metabolism MeSH
- Tyrosine 3-Monooxygenase metabolism MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Glial Fibrillary Acidic Protein MeSH
- Lipopolysaccharides MeSH
- STAT3 Transcription Factor MeSH
- STAT5 Transcription Factor MeSH
- Tyrosine 3-Monooxygenase MeSH
Signal transducers and activators of transcription (STAT) proteins regulate many aspects of cellular physiology from growth and differentiations to immune responses. Using immunohistochemistry, we show the daily rhythm of STAT3 protein in the rat suprachiasmatic nucleus (SCN), with low but significant amplitude peaking in the morning. We also reveal the strong expression of STAT5A in astrocytes of the SCN and the STAT5B signal in nonastrocytic cells. Administration of lipopolysaccharide (LPS) acutely induced phosphorylation of STAT3 on Tyr705 during both the day and the night and induced phosphorylation on Ser727 but only after the daytime application. The LPS-induced phospho-STAT3 (Tyr705) remained elevated for 24 hr after the daytime application but declined within 8 hr when LPS was applied at night.
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