Reward related neurotransmitter changes in a model of depression: An in vivo microdialysis study
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Depression, in vivo microdialysis, methamphetamine, olfactory bulbectomy, rats,
- MeSH
- Self Administration MeSH
- Depression drug therapy MeSH
- Dopamine metabolism MeSH
- Dopamine Agents administration & dosage MeSH
- gamma-Aminobutyric Acid metabolism MeSH
- Rats MeSH
- Glutamic Acid metabolism MeSH
- Methamphetamine administration & dosage MeSH
- Microdialysis MeSH
- Disease Models, Animal MeSH
- Neurotransmitter Agents metabolism MeSH
- Nucleus Accumbens physiopathology MeSH
- Reward * MeSH
- Rats, Wistar MeSH
- Serotonin metabolism MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Dopamine MeSH
- Dopamine Agents MeSH
- gamma-Aminobutyric Acid MeSH
- Glutamic Acid MeSH
- Methamphetamine MeSH
- Neurotransmitter Agents MeSH
- Serotonin MeSH
OBJECTIVES: The self-medication hypothesis assumes that symptoms related to potential monoaminergic deficits in depression may be relieved by drug abuse. The aim of this study was to elucidate the neurotransmitter changes in a rat model of depression by measuring their levels in the nucleus accumbens shell, which is typically involved in the drug of abuse acquisition mechanism. METHODS: Depression was modelled by the olfactory bulbectomy (OBX) in Wistar male rats. In vivo microdialysis was performed, starting from the baseline and following after a single methamphetamine injection and behaviour was monitored. The determination of neurotransmitters and their metabolites was performed by high-performance liquid chromatography combined with mass spectrometry. RESULTS: OBX animals had lower basal levels of dopamine and serotonin and their metabolites. However, γ-aminobutyric acid (GABA) and glutamate levels were increased. The methamphetamine injection induced stronger dopamine and serotonin release in the OBX rats and lower release of glutamate in comparison with sham-operated rats; GABA levels did not differ significantly. CONCLUSIONS: This study provides an evidence of mesolimbic neurotransmitter changes in the rat model of depression which may elucidate mechanisms underlying intravenous self-administration studies in which OBX rats were demonstrated to have higher drug intake in comparison to intact controls.
b Department of Pharmacology Faculty of Medicine Masaryk University Brno Czech Republic
c Department of Pharmacology 3rd Faculty of Medicine Charles University Prague Czech Republic and
Laboratory of Medicinal Diagnostics Department of Organic Technology ICT Prague Czech Republic
References provided by Crossref.org
The effect of CNQX on self-administration: present in nicotine, absent in methamphetamine model
