Reward related neurotransmitter changes in a model of depression: An in vivo microdialysis study
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Depression, in vivo microdialysis, methamphetamine, olfactory bulbectomy, rats,
- MeSH
- autoaplikace MeSH
- deprese farmakoterapie MeSH
- dopamin metabolismus MeSH
- dopaminové látky aplikace a dávkování MeSH
- GABA metabolismus MeSH
- krysa rodu Rattus MeSH
- kyselina glutamová metabolismus MeSH
- methamfetamin aplikace a dávkování MeSH
- mikrodialýza MeSH
- modely nemocí na zvířatech MeSH
- neurotransmiterové látky metabolismus MeSH
- nucleus accumbens patofyziologie MeSH
- odměna * MeSH
- potkani Wistar MeSH
- serotonin metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- dopamin MeSH
- dopaminové látky MeSH
- GABA MeSH
- kyselina glutamová MeSH
- methamfetamin MeSH
- neurotransmiterové látky MeSH
- serotonin MeSH
OBJECTIVES: The self-medication hypothesis assumes that symptoms related to potential monoaminergic deficits in depression may be relieved by drug abuse. The aim of this study was to elucidate the neurotransmitter changes in a rat model of depression by measuring their levels in the nucleus accumbens shell, which is typically involved in the drug of abuse acquisition mechanism. METHODS: Depression was modelled by the olfactory bulbectomy (OBX) in Wistar male rats. In vivo microdialysis was performed, starting from the baseline and following after a single methamphetamine injection and behaviour was monitored. The determination of neurotransmitters and their metabolites was performed by high-performance liquid chromatography combined with mass spectrometry. RESULTS: OBX animals had lower basal levels of dopamine and serotonin and their metabolites. However, γ-aminobutyric acid (GABA) and glutamate levels were increased. The methamphetamine injection induced stronger dopamine and serotonin release in the OBX rats and lower release of glutamate in comparison with sham-operated rats; GABA levels did not differ significantly. CONCLUSIONS: This study provides an evidence of mesolimbic neurotransmitter changes in the rat model of depression which may elucidate mechanisms underlying intravenous self-administration studies in which OBX rats were demonstrated to have higher drug intake in comparison to intact controls.
b Department of Pharmacology Faculty of Medicine Masaryk University Brno Czech Republic
c Department of Pharmacology 3rd Faculty of Medicine Charles University Prague Czech Republic and
Laboratory of Medicinal Diagnostics Department of Organic Technology ICT Prague Czech Republic
Citace poskytuje Crossref.org
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