SIMToolbox: a MATLAB toolbox for structured illumination fluorescence microscopy
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26446133
DOI
10.1093/bioinformatics/btv576
PII: btv576
Knihovny.cz E-resources
- MeSH
- Hep G2 Cells MeSH
- Fluorescence * MeSH
- Microscopy, Fluorescence methods MeSH
- Humans MeSH
- Actin Cytoskeleton ultrastructure MeSH
- Lighting methods MeSH
- Image Processing, Computer-Assisted MeSH
- Software * MeSH
- Imaging, Three-Dimensional methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
UNLABELLED: SIMToolbox is an open-source, modular set of functions for MATLAB equipped with a user-friendly graphical interface and designed for processing two-dimensional and three-dimensional data acquired by structured illumination microscopy (SIM). Both optical sectioning and super-resolution applications are supported. The software is also capable of maximum a posteriori probability image estimation (MAP-SIM), an alternative method for reconstruction of structured illumination images. MAP-SIM can potentially reduce reconstruction artifacts, which commonly occur due to refractive index mismatch within the sample and to imperfections in the illumination. AVAILABILITY AND IMPLEMENTATION: SIMToolbox, example data and the online documentation are freely accessible at http://mmtg.fel.cvut.cz/SIMToolbox. CONTACT: ghagen@uccs.edu SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
References provided by Crossref.org
Open-source microscope add-on for structured illumination microscopy
Super-resolution fluorescence microscopy by line-scanning with an unmodified two-photon microscope
