Biting midges of the genus Culicoides transmit pathogens of veterinary importance such as bluetongue virus (Reoviridae: Orbivirus). The saliva of Culicoides is known to contain bioactive molecules including peptides and proteins with vasodilatory and immunomodulative properties. In this study, we detected activity of enzyme hyaluronidase in six Culicoides species that commonly occur in Europe and that are putative vectors of arboviruses. Hyaluronidase was present in all species studied, although its molecular size, sensitivity to SDS, and substrate specificity differed between species. Further studies on the potential effect of hyaluronidase activity on the vector competence of Culicoides species for arboviruses would be beneficial.

Cirad UMR15 Contrôle des maladies Montpellier France INRA UMR1309 Contrôle des maladies Montpellier France

Department of Parasitology Charles University Prague Faculty of Science Czech Republic

Department of Parasitology Charles University Prague Faculty of Science Czech Republic Both authors contributed equally to this work

Vector borne Viral Diseases Programme The Pirbright Institute Pirbright Surrey GU24 0NF United Kingdom and

Vector borne Viral Diseases Programme The Pirbright Institute Pirbright Surrey GU24 0NF United Kingdom and School of Veterinary Medicine University of Surrey Guildford GU27AL United Kingdom

Zobrazit více v PubMed

Akey D. H., Luedke A. J., Jones R. H. 1985. Salivary gland homogenates from the vector PubMed

Boorman J. 1974. The maintenance of laboratory colonies of

Calvo E., Andersen J., Francischetti I. M., de L. Capurro M., deBianchi A. G., James A. A., Ribeiro J. M. C., Marinotti O. 2004. The transcriptome of adult female PubMed

Calvo E., Dao A., Pham V. M., Ribeiro J. M. C. 2007. An insight into the sialome of PubMed PMC

Campbell J. A., Pelham-Clinton E. C. 1960. A taxonomic review of the british species of

Campbell C. L., Vandyke K. A., Letchworth G. J., Drolet B. S., Hanekamp T., Wilson W. C. 2005. Midgut and salivary gland transcriptomes of the arbovirus vector PubMed

Černá P., Mikeš L., Volf P. 2002. Salivary gland hyaluronidase in various species of phlebotomine sand flies (Diptera: psychodidae). Insect Biochem. Mol. Biol. 32: 1691–1697. PubMed

Darpel K. E., Langner K. F. A., Nimtz M., Anthony S. J., Brownlie J., Takamatsu H.-H., Mellor P. S., Mertens P. P. C. 2011. Saliva proteins of vector PubMed PMC

Delécolle J.-C. 1985. Nouvelle contribution a` l’étude systématique et iconographique des espèces du genre

Dyce A. L. 1969. The recognition of nulliparous and parous

Hostomská J., Volfová V., Mu J., Garfield M., Rohousová I., Volf P., Valenzuela J. G., Jochim R. C. 2009. Analysis of salivary transcripts and antigens of the sand fly PubMed PMC

Huang Z., Zhao C., Chen Y., Cowell J. A., Wei G., Kultti A., Huang L., Thompson C. B., Rosengren S., Frost G. I., Shepard. H. M. 2014. Recombinant human hyaluronidase PH20 does not stimulate an acute inflammatory response and inhibits lipopolysaccharide-induced neutrophil recruitment in the air pouch model of inflammation. J. Immunol. 192: 5285–5295. PubMed

Charlab R., Valenzuela J. G., Rowton E. D., Ribeiro J. M. 1999. Toward an understanding of the biochemical and pharmacological complexity of the saliva of a hematophagous sand fly PubMed PMC

Chiarella P., De Santis S., Fazio V. M., Signori E. 2013. Hyaluronidase contributes to early inflammatory events induced by electrotransfer in mouse skeletal muscle. Hum. Gene Ther. 24: 406–416. PubMed

Langner K. F. A., Darpel K. E., Denison E., Drolet B. S., Leibold W., Mellor P. S., Mertens P.P.C., Nimtz M., Greiser-Wilke I. 2007. Collection and analysis of salivary proteins from the biting midge PubMed

Nayduch D., Cohnstaedt L. W., Saski C., Lawson D., Kersey P., Fife M., Carpenter S. 2014. Studying PubMed PMC

Nolan D. V., Dallas J. F., Mordue Luntz A. J. 2004. Molecular taxonomy and population structure of a PubMed

Pages N., Bréard E., Urien C., Talavera S., Viarouge C., Lorca-Oro C., Jouneau L., Charley B., Zientara S., Bensaid A., et al. 2014. PubMed PMC

Ribeiro J. M., Charlab R., Rowton E. D., Cupp E. W. 2000. PubMed

Ribeiro J. M. C., Arcà B., Lombardo F., Calvo E., Phan V. M., Chandra P. K., Wikel S. K. 2007. An annotated catalogue of salivary gland transcripts in the adult female mosquito, PubMed PMC

Rohoušová I., Subrahmanyam S., Volfová V., Mu J., Volf P., Valenzuela J. G., Jochim R. C. 2012. Salivary gland transcriptomes and proteomes of PubMed PMC

Russell C. L., Heesom K. J., Arthur C. J., Helps C. R., Mellor P. S., Day M. J., Torsteinsdottir S., Björnsdóttir T. S., Wilson A. D. 2009. Identification and isolation of cDNA clones encoding the abundant secreted proteins in the saliva proteome of PubMed

Schaffartzik A., Marti E., Torsteinsdottir S., Mellor P. S., Crameri R., Rhyner C. 2011. Selective cloning, characterization, and production of the PubMed

Termeer C., Sleeman J. P., Simon J. C. 2003. Hyaluronan – magic glue for the regulation of the immune response? Trends Immunol. 24: 112–114. PubMed

Van der Meide N. M. A., Roders N., Sloet van Oldruitenborgh-Oosterbaan M. M., Schaap P. J., van Oers M. M., Leibold W., Savelkoul H.F.J., Tijhaar E. 2013. Cloning and expression of candidate allergens from PubMed

Vlkova M., Sima M., Rohousova I., Kostalova T., Sumova P., Volfova V., Jaske E. L., Barbian K. D., Gebre-Michael T., Hailu A., et al. 2014. Comparative analysis of salivary gland transcriptomes of PubMed PMC

Volfova V., Hostomska J., Cerny M., Votypka J., Volf P. 2008. Hyaluronidase of bloodsucking insects and its enhancing effect on leishmania infection in mice. PLoS Negl. Trop. Dis. 2: e294. PubMed PMC

Wilson A. D., Harwood L. J., Björnsdottir S., Marti E., Day M. J. 2001. Detection of IgG and IgE serum antibodies to PubMed

Wilson A., Darpel K., Mellor P. S. 2008. Where does bluetongue virus sleep in the winter? PLoS Biol. 6: 1612–1617. PubMed PMC

Yeruham I., Braverman Y., Orgad U. 1993. Field observations in Israel on hypersensitivity in cattle, sheep and donkeys caused by PubMed

None

Human immune response against salivary antigens of Simulium damnosum s.l.: A new epidemiological marker for exposure to blackfly bites in onchocerciasis endemic areas