Bacteria as source of diglycosidase activity: Actinoplanes missouriensis produces 6-O-α-L-rhamnosyl-β-D-glucosidase active on flavonoids
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26549237
DOI
10.1007/s00253-015-7088-x
PII: 10.1007/s00253-015-7088-x
Knihovny.cz E-resources
- Keywords
- Inverting glycosidase, Methylumbelliferyl-β-D-rutinoside, α-L-rhamnosidase, β-D-glucosidase,
- MeSH
- Bacterial Proteins genetics metabolism MeSH
- beta-Glucosidase genetics metabolism MeSH
- Chalcones chemistry metabolism MeSH
- Disaccharides chemistry metabolism MeSH
- Escherichia coli genetics metabolism MeSH
- Gene Expression MeSH
- Flavonoids chemistry metabolism MeSH
- Phylogeny MeSH
- Glycosides chemistry metabolism MeSH
- Hesperidin analogs & derivatives chemistry metabolism MeSH
- Hydrolysis MeSH
- Cloning, Molecular MeSH
- Micromonosporaceae classification genetics metabolism MeSH
- Recombinant Proteins genetics metabolism MeSH
- Rhamnose chemistry metabolism MeSH
- Substrate Specificity MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bacterial Proteins MeSH
- beta-Glucosidase MeSH
- Chalcones MeSH
- Disaccharides MeSH
- Flavonoids MeSH
- Glycosides MeSH
- hesperidin methylchalcone MeSH Browser
- Hesperidin MeSH
- Recombinant Proteins MeSH
- Rhamnose MeSH
- rutinose MeSH Browser
Bacteria represent an underexplored source of diglycosidases. Twenty-five bacterial strains from the genera Actinoplanes, Bacillus, Corynebacterium, Microbacterium, and Streptomyces were selected for their ability to grow in diglycosylated flavonoids-based media. The strains Actinoplanes missouriensis and Actinoplanes liguriae exhibited hesperidin deglycosylation activity (6-O-α-L-rhamnosyl-β-D-glucosidase activity, EC 3.2.1.168), which was 3 to 4 orders of magnitude higher than the corresponding monoglycosidase activities. The diglycosidase production was confirmed in A. missouriensis by zymographic assays and NMR analysis of the released disaccharide, rutinose. The gene encoding the 6-O-α-L-rhamnosyl-β-D-glucosidase was identified in the genome sequence of A. missouriensis 431(T) (GenBank accession number BAL86042.1) and functionally expressed in Escherichia coli. The recombinant protein hydrolyzed hesperidin and hesperidin methylchalcone, but not rutin, which indicates its specificity for 7-O-rutinosylated flavonoids. The protein was classified into the glycoside hydrolase family 55 (GH55) in contrast to the known eukaryotic diglycosidases, which belong to GH1 and GH5. These findings demonstrate that organisms other than plants and filamentous fungi can contribute to an expansion of the diglycosidase toolbox.
References provided by Crossref.org
Flavonoids as Aglycones in Retaining Glycosidase-Catalyzed Reactions: Prospects for Green Chemistry
"Sweet Flavonoids": Glycosidase-Catalyzed Modifications
