Evaluation of 3-hydroxybutyrate as an enzyme-protective agent against heating and oxidative damage and its potential role in stress response of poly(3-hydroxybutyrate) accumulating cells
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26590589
DOI
10.1007/s00253-015-7162-4
PII: 10.1007/s00253-015-7162-4
Knihovny.cz E-resources
- Keywords
- 3-Hydroxybutyrate, Chemical chaperone, Compatible solutes, PHB, PHB cycle, Poly(3-hydroxybutyrate),
- MeSH
- Bacterial Proteins chemistry metabolism MeSH
- Cupriavidus necator chemistry enzymology metabolism MeSH
- Hydroxybutyrates metabolism MeSH
- 3-Hydroxybutyric Acid chemistry metabolism MeSH
- Muramidase chemistry metabolism MeSH
- Protective Agents chemistry metabolism MeSH
- Oxidation-Reduction MeSH
- Oxidative Stress MeSH
- Polyesters metabolism MeSH
- Enzyme Stability MeSH
- Hot Temperature MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bacterial Proteins MeSH
- Hydroxybutyrates MeSH
- 3-Hydroxybutyric Acid MeSH
- Muramidase MeSH
- Protective Agents MeSH
- poly-beta-hydroxybutyrate MeSH Browser
- Polyesters MeSH
Poly(3-hydroxybutyrate) (PHB) is a common carbon- and energy-storage compound simultaneously produced and degraded into its monomer 3-hydroxybutyrate (3HB) by numerous bacteria and Archae in a metabolic pathway called the PHB cycle. We investigated 3HB as a chemical chaperone capable of protecting model enzymes, namely lipase and lysozyme, from adverse effects of high temperature and oxidation. Heat-mediated denaturation of lipase in the presence or absence of 3HB was monitored by dynamic light scattering (DLS) revealing a significant protective effect of 3HB which increased as its concentration rose. Furthermore, when compared at the same molar concentration, 3HB showed a greater protective effect than the well-known chemical chaperones trehalose and hydroxyectoine. The higher protective effect of 3HB was also confirmed when employing differential scanning calorimetry (DSC) and lysozyme as a model enzyme. Furthermore, 3HB was capable of protecting lipase not only against thermal-mediated denaturation but also against oxidative damage by Cu(2+) and H2O2; its protection was higher than that of trehalose and comparable to that of hydroxyectoine. Taking into account that the PHB-producing strain Cupriavidus necator H16 reveals a 16.5-fold higher intracellular concentration than the PHB non-producing mutant C. necator PHB(-4), it might be expected that the functional PHB cycle might be responsible for maintaining a higher intracellular level of 3HB which, aside from other positive aspects of functional PHB metabolism, enhances stress resistance of bacterial strains capable of simultaneous PHB synthesis and mobilization. In addition, 3HB can be used in various applications and formulations as an efficient enzyme-stabilizing and enzyme-protecting additive.
References provided by Crossref.org
On the bioprotective effects of 3-hydroxybutyrate: Thermodynamic study of binary 3HB-water systems
Accumulation of Poly(3-hydroxybutyrate) Helps Bacterial Cells to Survive Freezing
