Signs of myelin impairment in cerebrospinal fluid after osmotic opening of the blood-brain barrier in rats
Language English Country Czech Republic Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26674284
DOI
10.33549/physiolres.933220
PII: 933220
Knihovny.cz E-resources
- MeSH
- Biomarkers cerebrospinal fluid MeSH
- Myelin Basic Protein cerebrospinal fluid MeSH
- Phosphopyruvate Hydratase cerebrospinal fluid MeSH
- Blood-Brain Barrier drug effects metabolism pathology MeSH
- Mannitol toxicity MeSH
- Myelin Sheath drug effects metabolism pathology MeSH
- Nerve Fibers, Myelinated drug effects metabolism pathology MeSH
- Osmotic Pressure MeSH
- Permeability MeSH
- Rats, Wistar MeSH
- tau Proteins cerebrospinal fluid MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biomarkers MeSH
- Myelin Basic Protein MeSH
- Phosphopyruvate Hydratase MeSH
- Mannitol MeSH
- Mapt protein, rat MeSH Browser
- Mbp protein, rat MeSH Browser
- tau Proteins MeSH
A number of clinical neurological pathologies are associated with increased permeability of the blood brain barrier (BBB). Induced changes of the homeostatic mechanisms in the brain microenvironment lead among others to cellular changes in the CNS. The question was whether some of these changes can be induced by osmotic opening of BBB in an in vivo experiment and whether they can be detected in cerebrospinal fluid (CSF). CSF was taken via the suboccipital puncture from 10 healthy rats and six rats after the osmotic opening of the BBB. In all 16 animals, concentration of myelin basic protein (MBP ng/ml), Neuron-specific enolase (NSE ng/ml) and Tau-protein (Tau pg/ml) were determined in CSF by ELISA. Values in both groups were statistically evaluated. Significant difference between the control and experimental group was revealed only for the concentration of myelin basic protein (p<0.01). The presented results indicate that osmotic opening of the BBB in vivo experiment without the presence of other pathological conditions of the brain leads to a damage of myelin, without impairment of neurons or their axons.
References provided by Crossref.org
Effect of methylprednisolone on experimental brain edema in rats - own experience reviewed
Effect of methylprednisolone on experimental brain edema in magnetic resonance imaging
