C2-substituted aromatic cytokinin sugar conjugates delay the onset of senescence by maintaining the activity of the photosynthetic apparatus
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26706318
DOI
10.1016/j.phytochem.2015.12.001
PII: S0031-9422(15)30119-9
Knihovny.cz E-zdroje
- Klíčová slova
- Aromatic cytokinin, Cytokinin activity, Gene expression, Photosynthesis, Photosystem, Ribosides, Senescence delay, Synthesis,
- MeSH
- amarant metabolismus MeSH
- Arabidopsis metabolismus MeSH
- cytokininy metabolismus MeSH
- fotosyntetické reakční centrum - proteinové komplexy fyziologie MeSH
- listy rostlin metabolismus MeSH
- metabolismus sacharidů fyziologie MeSH
- pšenice metabolismus MeSH
- purinové nukleosidy chemická syntéza chemie MeSH
- ribonukleosidy chemická syntéza chemie MeSH
- stárnutí účinky léků fyziologie MeSH
- tabák metabolismus MeSH
- vývoj rostlin účinky léků MeSH
- vztahy mezi strukturou a aktivitou MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cytokininy MeSH
- fotosyntetické reakční centrum - proteinové komplexy MeSH
- nebularine MeSH Prohlížeč
- purinové nukleosidy MeSH
- ribonukleosidy MeSH
Cytokinins are plant hormones with biological functions ranging from coordination of plant growth and development to the regulation of senescence. A series of 2-chloro-N(6)-(halogenobenzylamino)purine ribosides was prepared and tested for cytokinin activity in detached wheat leaf senescence, tobacco callus and Amaranthus bioassays. The synthetic compounds showed significant activity, especially in delaying senescence in detached wheat leaves. They were also tested in bacterial receptor bioassays using both monocot and dicot members of the cytokinin receptor family. Most of the derivatives did not trigger cytokinin signaling via the AHK3 and AHK4 receptors from Arabidopsis thaliana in the bacterial assay, but some of them specifically activated the ZmHK1 receptor from Zea mays and were also more active than the aromatic cytokinin BAP in an ARR5::GUS cytokinin bioassay using transgenic Arabidopsis plants. Whole transcript expression analysis was performed using an Arabidopsis model to gather information about the reprogramming of gene transcription when senescent leaves were treated with selected C2-substituted aromatic cytokinin ribosides. Genome-wide expression profiling revealed that the synthetic halogenated derivatives induced the expression of genes related to cytokinin signaling and metabolism. They also prompted both up- and down-regulation of a unique combination of genes coding for components of the photosystem II (PSII) reaction center, light-harvesting complex II (LHCII), and the oxygen-evolving complex, as well as several stress factors responsible for regulating photosynthesis and chlorophyll degradation. Chlorophyll content and fluorescence analyses demonstrated that treatment with the halogenated derivatives increased the efficiency of PSII photochemistry and the abundance of LHCII relative to DMSO- and BAP-treated controls. These findings demonstrate that it is possible to manipulate and fine-tune leaf longevity using synthetic aromatic cytokinin analogs.
Citace poskytuje Crossref.org
Plant hormone cytokinin at the crossroads of stress priming and control of photosynthesis
Role of Cytokinins in Senescence, Antioxidant Defence and Photosynthesis
Cytokinin-Specific Glycosyltransferases Possess Different Roles in Cytokinin Homeostasis Maintenance
