A quantitative evaluation of ethylene production in the recombinant cyanobacterium Synechocystis sp. PCC 6803 harboring the ethylene-forming enzyme by membrane inlet mass spectrometry
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26708481
DOI
10.1016/j.biortech.2015.11.062
PII: S0960-8524(15)01595-3
Knihovny.cz E-resources
- Keywords
- Biofuels, Biotechnology, Cyanobacteria, MIMS, Photobioreactor,
- MeSH
- Autotrophic Processes MeSH
- Ethylenes biosynthesis MeSH
- Mass Spectrometry instrumentation methods MeSH
- Oxygen analysis MeSH
- Lyases metabolism MeSH
- Membranes, Artificial * MeSH
- Metabolic Networks and Pathways MeSH
- Recombination, Genetic genetics MeSH
- Light MeSH
- Synechocystis enzymology growth & development radiation effects MeSH
- Carbon analysis MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- ethylene forming enzyme MeSH Browser
- ethylene MeSH Browser
- Ethylenes MeSH
- Oxygen MeSH
- Lyases MeSH
- Membranes, Artificial * MeSH
- Carbon MeSH
The prediction of the world's future energy consumption and global climate change makes it desirable to identify new technologies to replace or augment fossil fuels by environmentally sustainable alternatives. One appealing sustainable energy concept is harvesting solar energy via photosynthesis coupled to conversion of CO2 into chemical feedstock and fuel. In this work, the production of ethylene, the most widely used petrochemical produced exclusively from fossil fuels, in the model cyanobacterium Synechocystis sp. PCC 6803 is studied. A novel instrumentation setup for quantitative monitoring of ethylene production using a combination of flat-panel photobioreactor coupled to a membrane-inlet mass spectrometer is introduced. Carbon partitioning is estimated using a quantitative model of cyanobacterial metabolism. The results show that ethylene is produced under a wide range of light intensities with an optimum at modest irradiances. The results allow production conditions to be optimized in a highly controlled setup.
Department of Life Sciences Imperial College London London United Kingdom
Institut für Theoretische Biologie Humboldt Universität zu Berlin Berlin Germany
References provided by Crossref.org
A quantitative description of light-limited cyanobacterial growth using flux balance analysis
Towards a quantitative assessment of inorganic carbon cycling in photosynthetic microorganisms
Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
Optimizing cyanobacterial product synthesis: Meeting the challenges
