To study the mechanisms involved in the maintenance of a linear mitochondrial genome we investigated the biochemical properties of the recombination protein Mgm101 from Candida parapsilosis. We show that CpMgm101 complements defects associated with the Saccharomyces cerevisiae mgm101-1(ts) mutation and that it is present in both the nucleus and mitochondrial nucleoids of C. parapsilosis. Unlike its S. cerevisiae counterpart, CpMgm101 is associated with the entire nucleoid population and is able to bind to a broad range of DNA substrates in a non-sequence specific manner. CpMgm101 is also able to catalyze strand annealing and D-loop formation. CpMgm101 forms a roughly C-shaped trimer in solution according to SAXS. Electron microscopy of a complex of CpMgm101 with a model mitochondrial telomere revealed homogeneous, ring-shaped structures at the telomeric single-stranded overhangs. The DNA-binding properties of CpMgm101, together with its DNA recombination properties, suggest that it can play a number of possible roles in the replication of the mitochondrial genome and the maintenance of its telomeres.

Department for Structural and Computational Biology Max F Perutz Laboratories Dr Bohr Gasse 9 1030 Vienna Austria

Department of Biochemistry and Structural Biology Institute of Molecular Biology Slovak Academy of Sciences Dúbravská cesta 21 845 51 Bratislava Slovakia

Department of Biochemistry and Structural Biology Institute of Molecular Biology Slovak Academy of Sciences Dúbravská cesta 21 845 51 Bratislava Slovakia Institute of Microbiology of the CAS v v i 142 20 Prague Czech Republic

Department of Biochemistry Faculty of Natural Sciences Comenius University Mlynská dolina CH 1 Ilkovičova 6 842 15 Bratislava Slovakia

Department of Biology Masaryk University Kamenice 5 625 00 Brno Czech Republic National Centre for Biomolecular Research Masaryk University Kamenice 5 625 00 Brno Czech Republic

Department of Genetics Faculty of Natural Sciences Comenius University Mlynská dolina B 1 Ilkovičova 6 842 15 Bratislava Slovakia

Electron Microscopy Facility of the Campus Science Support Facilities GmbH Dr Bohr Gasse 3 1030 Vienna Austria

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