The level and distribution pattern of HP1β in the embryonic brain correspond to those of H3K9me1/me2 but not of H3K9me3
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26794325
DOI
10.1007/s00418-015-1402-7
PII: 10.1007/s00418-015-1402-7
Knihovny.cz E-resources
- Keywords
- Brain sections, Epigenetics, Hippocampus, Histone methylation, Histones, Olfactory bulb,
- MeSH
- Chromosomal Proteins, Non-Histone analysis metabolism MeSH
- Microscopy, Fluorescence MeSH
- Histone-Lysine N-Methyltransferase metabolism MeSH
- Immunohistochemistry MeSH
- Brain embryology metabolism MeSH
- Mice, Inbred C57BL MeSH
- Mice, Knockout MeSH
- Mice MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cbx1 protein, mouse MeSH Browser
- Chromosomal Proteins, Non-Histone MeSH
- Histone-Lysine N-Methyltransferase MeSH
We studied the histone signature of embryonic and adult brains to strengthen existing evidence of the importance of the histone code in mouse brain development. We analyzed the levels and distribution patterns of H3K9me1, H3K9me2, H3K9me3, and HP1β in both embryonic and adult brains. Western blotting showed that during mouse brain development, the levels of H3K9me1, H3K9me2, and HP1β exhibited almost identical trends, with the highest protein levels occurring at E15 stage. These trends differed from the relatively stable level of H3K9me3 at developmental stages E8, E13, E15, and E18. Compared with embryonic brains, adult brains were characterized by very low levels of H3K9me1/me2/me3 and HP1β. Manipulation of the embryonic epigenome through histone deacetylase inhibitor treatment did not affect the distribution patterns of the studied histone markers in embryonic ventricular ependyma. Similarly, Hdac3 depletion in adult animals had no effect on histone methylation in the adult hippocampus. Our results indicate that the distribution of HP1β in the embryonic mouse brain is related to that of H3K9me1/me2 but not to that of H3K9me3. The unique status of H3K9me3 in the brain was confirmed by its pronounced accumulation in the granular layer of the adult olfactory bulb. Moreover, among the studied proteins, H3K9me3 was the only posttranslational histone modification that was highly abundant at clusters of centromeric heterochromatin, called chromocenters. When we focused on the hippocampus, we found this region to be rich in H3K9me1 and H3K9me3, whereas H3K9me2 and HP1β were present at a very low level or even absent in the hippocampal blade. Taken together, these results revealed differences in the epigenome of the embryonic and adult mouse brain and showed that the adult hippocampus, the granular layer of the adult olfactory bulb, and the ventricular ependyma of the embryonic brain are colonized by specific epigenetic marks.
See more in PubMed
Mol Cell. 2007 Aug 17;27(4):585-95 PubMed
Bioessays. 2004 Aug;26(8):821-4 PubMed
Histochem Cell Biol. 2007 Apr;127(4):375-88 PubMed
Development. 1989 Feb;105(2):365-78 PubMed
Genome Biol. 2010;11(7):R79 PubMed
Mol Cell Biol. 2005 Apr;25(7):2525-38 PubMed
J Cell Sci. 2005 Nov 1;118(Pt 21):5035-46 PubMed
J Cell Biol. 2008 Nov 17;183(4):597-606 PubMed
Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13541-6 PubMed
PLoS One. 2012;7(2):e31080 PubMed
Genes Dev. 2011 Apr 15;25(8):781-8 PubMed
Neurosci Lett. 1995 Sep 29;198(2):131-4 PubMed
Cell. 2006 Apr 21;125(2):315-26 PubMed
Nat Neurosci. 2010 Nov;13(11):1338-44 PubMed
Cell Stem Cell. 2012 Jun 14;10(6):698-708 PubMed
J Cell Sci. 2002 Dec 1;115(Pt 23):4433-45 PubMed
J Neurosci. 2002 May 1;22(9):3520-30 PubMed
Cell Cycle. 2011 Feb 15;10(4):625-30 PubMed
Genes Dev. 2010 Oct 1;24(19):2133-45 PubMed
Chromosoma. 2010 Jun;119(3):227-41 PubMed
Cold Spring Harb Symp Quant Biol. 2010;75:71-8 PubMed
Cell. 2007 Feb 23;128(4):693-705 PubMed
Genes Dev. 2008 Apr 1;22(7):832-53 PubMed
Cell. 2012 Aug 31;150(5):934-47 PubMed
Stem Cells. 2006 Jun;24(6):1594-604 PubMed
Epigenetics Chromatin. 2014 Dec 30;7(1):39 PubMed
Cell. 2012 Aug 31;150(5):948-60 PubMed
Genes Dev. 2015 Feb 15;29(4):379-93 PubMed
Science. 2015 Apr 3;348(6230):132-5 PubMed
Clin Epigenetics. 2014 Oct 27;6(1):21 PubMed
J Biol Chem. 2014 Dec 12;289(50):34569-82 PubMed
Arch Gen Psychiatry. 1998 May;55(5):433-40 PubMed
J Struct Biol. 2010 Jan;169(1):124-33 PubMed
Blood. 2000 Mar 1;95(5):1608-15 PubMed
Nat Rev Mol Cell Biol. 2005 Nov;6(11):838-49 PubMed
Epigenomics. 2013 Aug;5(4):379-96 PubMed
Nat Commun. 2014 Dec 18;5:5868 PubMed
Cell. 1987 Nov 6;51(3):383-92 PubMed
Nat Cell Biol. 2001 Feb;3(2):114-20 PubMed
Genes Dev. 2006 Jun 15;20(12):1557-62 PubMed
Cell differentiation and aging accompanied by depletion of the ACE2 protein
Polyaniline cryogels: Biocompatibility of novel conducting macroporous material
