Transcripts of genes encoding reproductive neuroendocrine hormones and androgen receptor in the brain and testis of goldfish exposed to vinclozolin, flutamide, testosterone, and their combinations
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
26899179
DOI
10.1007/s10695-016-0205-7
PII: 10.1007/s10695-016-0205-7
Knihovny.cz E-resources
- Keywords
- Androgen receptor, Flutamide, Kisspeptin, Luteinizing hormone receptor, Salmon gonadotropin-releasing hormone, Testosterone,
- MeSH
- Receptors, Androgen genetics MeSH
- Androgens pharmacology MeSH
- Androgen Antagonists pharmacology MeSH
- Flutamide pharmacology MeSH
- Gonadotropin-Releasing Hormone genetics MeSH
- Goldfish genetics MeSH
- Kisspeptins genetics MeSH
- Pyrrolidonecarboxylic Acid analogs & derivatives MeSH
- Drug Interactions MeSH
- RNA, Messenger metabolism MeSH
- Brain drug effects metabolism MeSH
- Oxazoles pharmacology MeSH
- Fungicides, Industrial pharmacology MeSH
- Receptors, LH genetics MeSH
- Receptors, G-Protein-Coupled genetics MeSH
- Reproduction drug effects MeSH
- Fish Proteins genetics MeSH
- Testis drug effects metabolism MeSH
- Testosterone pharmacology MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Receptors, Androgen MeSH
- Androgens MeSH
- Androgen Antagonists MeSH
- Flutamide MeSH
- gonadotropin-releasing hormone-III MeSH Browser
- Gonadotropin-Releasing Hormone MeSH
- Kisspeptins MeSH
- Pyrrolidonecarboxylic Acid MeSH
- RNA, Messenger MeSH
- Oxazoles MeSH
- Fungicides, Industrial MeSH
- Receptors, LH MeSH
- Receptors, G-Protein-Coupled MeSH
- Fish Proteins MeSH
- Testosterone MeSH
- vinclozolin MeSH Browser
Vinclozolin (VZ) is a pesticide that acts as an anti-androgen to impair reproduction in mammals. However, VZ-induced disruption of reproduction is largely unknown in fish. In the present study, we have established a combination exposure in which adult goldfish were exposed to VZ (30 and 100 μg/L), anti-androgen flutamide (Flu, 300 μg/L), and androgen testosterone (T, 1 μg/L) to better understand effects of VZ on reproductive endocrine system. mRNA levels of kisspeptin (kiss-1 and kiss-2) and its receptor (gpr54), salmon gonadotropin-releasing hormone (gnrh3) and androgen receptor (ar) in the mid-brain, and luteinizing hormone receptor (lhr) in the testis were analyzed and compared with those of control following 10 days of exposure. kiss-1 mRNA level was increased in goldfish exposed to 100 µg/L VZ and to Flu, while kiss-2 mRNA level was increased following exposure to Flu and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. gpr54 mRNA level was increased in goldfish exposed to Flu and to combination of 30 µg/L VZ with Flu and 100 µg/L VZ with T. gnrh3 mRNA level was increased in goldfish exposed to 100 µg/L VZ, to Flu, and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. The mid-brain ar mRNA level was increased in goldfish exposed to Flu and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. Testicular lhr mRNA level was increased in goldfish exposed to Flu and to combination of 30 µg/L VZ with Flu. These results suggest that VZ and Flu are capable of interfering with kisspeptin and GnRH systems to alter pituitary and testicular horonal functions in adult goldfish and the brain ar mediates VZ-induced disruption of androgen production.
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Toxicol Ind Health. 1999 Jan-Mar;15(1-2):48-64 PubMed
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Endocrinology. 1994 Feb;134(2):838-47 PubMed
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