Expression of a second open reading frame present in the genome of tick-borne encephalitis virus strain Neudoerfl is not detectable in infected cells
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
26924586
DOI
10.1007/s11262-015-1273-y
PII: 10.1007/s11262-015-1273-y
Knihovny.cz E-zdroje
- Klíčová slova
- Immunoblotting, Immunofluorescence, TBEV, TuORF, uORF,
- MeSH
- biosyntéza peptidů genetika imunologie MeSH
- buněčné linie MeSH
- fylogeneze MeSH
- genom virový * MeSH
- glioblastom virologie MeSH
- klíště virologie MeSH
- klíšťová encefalitida virologie MeSH
- lidé MeSH
- meduloblastom virologie MeSH
- mutace MeSH
- neuroblastom virologie MeSH
- otevřené čtecí rámce * MeSH
- viry klíšťové encefalitidy genetika MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
A short upstream open reading frame (uORF) was recently identified in the 5' untranslated region of some tick-borne encephalitis virus (TBEV) strains. However, it is not known if the peptide encoded by TBEV uORF (TuORF) is expressed in infected cells. Here we show that TuORF forms three phylogenetically separated clades which are typical of European, Siberian, and Far-Eastern TBEV subtypes. Analysis of selection pressure acting on the TuORF area showed that it is under positive selection pressure. Theoretically, TuORF may code for a short hydrophobic peptide embedded in a biological membrane. However, expression of TuORF was detectable neither by immunoblotting in tick and mammalian cell lines infected with TBEV nor by immunofluorescence in TBEV-infected mammalian cell lines. These results support the idea that TuORF is not expressed in TBEV-infected cell or expressed in undetectably low concentrations. Therefore we can assume that TuORF has either minor or no biological role in the TBEV life cycle.
The Pirbright Institute Ash Road Pirbright Woking Surrey GU24 0NF UK
Veterinary Research Institute Hudcova 296 70 621 00 Brno Czech Republic
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