The cutting of ultrathin sections with the thickness less than 20 nm from biological specimens embedded in resin blocks
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
27030160
DOI
10.1002/jemt.22659
Knihovny.cz E-resources
- Keywords
- low voltage electron microscopy, resin embedding, ultramicrotomy, ultrathin sectioning,
- MeSH
- Equipment Design MeSH
- Microscopy, Electron instrumentation methods MeSH
- Epoxy Resins chemistry MeSH
- Microtomy methods MeSH
- Myocardium ultrastructure MeSH
- Mice MeSH
- Polymers chemistry MeSH
- Heart diagnostic imaging MeSH
- Plastic Embedding methods MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Epoxy Resins MeSH
- Polybed 812 MeSH Browser
- Polymers MeSH
- spurr resin MeSH Browser
Low voltage electron microscopes working in transmission mode, like LVEM5 (Delong Instruments, Czech Republic) working at accelerating voltage 5 kV or scanning electron microscope working in transmission mode with accelerating voltage below 1 kV, require ultrathin sections with the thickness below 20 nm. Decreasing of the primary electron energy leads to enhancement of image contrast, which is especially useful in the case of biological samples composed of elements with low atomic numbers. As a result treatments with heavy metals, like post-fixation with osmium tetroxide or ultrathin section staining, can by omitted. The disadvantage is reduced penetration ability of incident electrons influencing the usable thickness of the specimen resulting in the need of ultrathin sections of under 20 nm thickness. In this study we want to answer basic questions concerning the cutting of extremely ultrathin sections: Is it possible routinely and reproducibly to cut extremely thin sections of biological specimens embedded in commonly used resins with contemporary ultramicrotome techniques and under what conditions? Microsc. Res. Tech. 79:512-517, 2016. © 2016 Wiley Periodicals, Inc.
Delong Instruments a s Palackého třída 3019 Brno 612 00 Czech Republic
Faculty of Science Charles University Prague Viničná 7 128 08 Praha 2 Czech Republic
References provided by Crossref.org
