Rapid assignment of malting barley varieties by matrix-assisted laser desorption-ionisation - Time-of-flight mass spectrometry
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
27041307
DOI
10.1016/j.foodchem.2016.03.056
PII: S0308-8146(16)30409-5
Knihovny.cz E-resources
- Keywords
- Barley, MALDI-TOF MS, Malting, Proteins,
- MeSH
- Glutens analysis MeSH
- Hordeum chemistry classification metabolism MeSH
- Beer MeSH
- Food-Processing Industry methods MeSH
- Seedlings chemistry classification metabolism MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Glutens MeSH
A method for discriminating malting barley varieties based on direct matrix-assisted laser desorption-ionisation - time-of-flight mass spectrometry (MALDI-TOF MS) fingerprinting of proteins was developed. Signals corresponding to hordeins were obtained by simple mixing of powdered barley grain with a MALDI matrix solution containing 12.5mgmL(-1) of ferulic acid in an acetonitrile:water:formic acid 50:33:17 v/v/v mixture. Compared to previous attempts at MALDI-TOF mass spectrometric analysis of barley proteins, the extraction and fractionation steps were practically omitted, resulting in a significant reduction in analytical time and costs. The discriminatory power was examined on twenty malting barley varieties and the practicability of the method was tested on sixty barley samples acquired from Pilsner Urquell Brewery. The method is proposed as a rapid tool for variety assignment and purity determination of malting barley that may replace gel electrophoresis currently used for this purpose.
Bruker Daltonics s r o Pražákova 60 61900 Brno Czech Republic
Pilsner Urquell Brewery U prazdroje 7 30497 Plzeň Czech Republic
References provided by Crossref.org
Biomolecular Profiling by MALDI-TOF Mass Spectrometry in Food and Beverage Analyses
