The Supportive Role of Insulin-Like Growth Factor-I in the Differentiation of Murine Mesenchymal Stem Cells into Corneal-Like Cells
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
27050039
DOI
10.1089/scd.2016.0030
Knihovny.cz E-resources
- MeSH
- Biomarkers metabolism MeSH
- Cell Differentiation drug effects genetics MeSH
- Immunosuppression Therapy MeSH
- Insulin-Like Growth Factor I pharmacology MeSH
- Mesenchymal Stem Cells cytology drug effects metabolism MeSH
- Mice, Inbred BALB C MeSH
- Cell Proliferation drug effects genetics MeSH
- Gene Expression Regulation drug effects MeSH
- Cornea cytology MeSH
- Cell Shape drug effects genetics MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Biomarkers MeSH
- Insulin-Like Growth Factor I MeSH
This study was focused on characterizing the differentiation of bone marrow-derived mesenchymal stem cells (MSCs) into corneal-like cells. Mouse MSCs were isolated from the bone marrow, grown in cell culture for 3 weeks, and purified using a magnetic activated cell sorter. Purified MSCs were cultured with an extract prepared from excised corneas and in the presence or absence of insulin-like growth factor-I (IGF-I). Analysis by quantitative real-time polymerase chain reaction showed that the expression of corneal specific markers, such as cytokeratin 12 (K12), keratocan, and lumican, was already induced after a 3-day cultivation and gradually increased during the 10-day incubation of MSCs with the extract. The presence of IGF-I significantly increased differentiation. Immunofluorescence analysis of differentiated MSCs showed positive results for the K12 protein. The morphology of the differentiated cells and the expression of cell surface markers CD45, CD11b, CD73, CD44, and CD105 were comparable in the control and differentiated MSCs. Proliferative activity was even higher in differentiated cells than in untreated MSCs. Both untreated and differentiated MSCs inhibited the production of interleukin-2 and interferon-γ in spleen cells stimulated with Concanavalin A. The results thus show that MSCs cultured in the presence of corneal extract and IGF-I efficiently differentiate into corneal-like cells. The differentiated cells possess characteristics of corneal epithelial cells and keratocytes, while at the same time maintaining MSC properties.
References provided by Crossref.org
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Interleukin-13 increases the stemness of limbal epithelial stem cells cultures
The Immunomodulatory Potential of Mesenchymal Stem Cells in a Retinal Inflammatory Environment
